{"product_id":"biolegend-906110","title":"Biolegend, 906110, Direct-Blot™ HRP anti-6-His Epitope Tag Antibody, 100microl","description":"\u003cp\u003eHis tags are commonly used as a method of tagging proteins for detection or purification. This antibody is raised again a polyhistidine tag (6-His or hexa His-tag).\u003cbr\u003e\n100microl\u003cbr\u003e\nVerified Reactivity: Epitope tag\u003cbr\u003e\nReported Reactivity: Species independent\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Monoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH.\u003cbr\u003e\nPurity: The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.\u003cbr\u003e\nFormulation: This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.05 - 5.0 µg per ml (1:1000-1:10000 dilution). It is recommended that the reagent be titrated for optimal performance for each application. 25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration\/dilution.\u003cbr\u003e\nApplication Notes: The sequence of the protein flanking the poly His tag does not influence antibody binding. This antibody was subsequently purified via a protein-G column.\u003cbr\u003e\nApplication References(PubMed link indicates BioLegend citation): Deinert K, et al. 2001. J. Biol. Chem. 276:6000. (WB) Kojima H, et al. 2001. J. Biol. Chem.(22)276:19238. (ICC) PubMed Lapinski PE, et al. 2000. J. Biol. Chem. 275:6831. (WB) Bartsch D, et al. 1998. Cell. 95:211. (ICC) Fuentes EJ, et al. 2003. J Biol Chem. 278:2118. PubMed Montegna EA, et al. 2012 PLoS One.7:31156. (WB) PubMed Huang L, et al. 2013. Nat Biotechnol. 31:450. (WB) PubMed\u003cbr\u003e\nProduct Citations: Chandrasekar SS, et al. 2021. Vaccines (Basel). 9:. PubMed\u003cbr\u003e\nRRID: AB_2734681 (BioLegend Cat. No. 906109) AB_2734682 (BioLegend Cat. No. 906110)\u003cbr\u003e\nBiology Area: Cell Biology\u003cbr\u003e\nGene ID: NA\u003cbr\u003e\nUniProt: View information about 6-His on UniProt.org\u003cbr\u003e\nClone: 6-His\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: polyhistidine-tag, hexa histidine-tag, 6xHis-tag, His6 tag, His-tag(TM)\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862464909481,"sku":"906110","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-906110","provider":"Iright","version":"1.0","type":"link"}