{"product_id":"biolegend-933401","title":"Biolegend, 933401, Purified anti-Rb Antibody, 25μg","description":"\u003cp\u003eRb is a key regulator of the cell cycle, more specifically during the transition from the G1 to S phases. The underphosphorylated, active form of Rb interacts directly with E2F1, leading to cell cycle arrest, while the hyperphosphorylated form decouples from E2F1, thus promoting the transcription of genes promoting entry into the S phase. Rb is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant human Rb protein.\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IP, ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 µg\/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg\/mL. For immunocytochemistry, a concentration of 5 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nRRID: AB_2820205 (BioLegend Cat. No. 933401) AB_2820206 (BioLegend Cat. No. 933402)\u003cbr\u003e\nStructure: Rb is a 928 amino-acid protein with a predicted molecular weight of 110 kD.\u003cbr\u003e\nDistribution: Localized to the nucleoplasm\/Ubiquitously expressed.\u003cbr\u003e\nFunction: Regulator; tumor suppressor; chromatin stability\u003cbr\u003e\nInteraction: E2F1 Transcription Factor\u003cbr\u003e\nBiology Area: Apoptosis\/Tumor Suppressors\/Cell Death, Cell Cycle\/DNA Replication\u003cbr\u003e\nMolecular Family: Tumor Suppressors\u003cbr\u003e\nAntigen References: Dunham A, et al. 2004. Nature. 428(6982):522-8. Hogg A, et al. 1992. Oncogene. (7):1445-51. Cho HS, et al. 2012. Neoplasia. 14(6):476-86. Mori T, et al. 2011. Cell Cycle. 10(19):3284-99. Rose JE, et al. 2010. Mol Med. 16(7-8):247-53.\u003cbr\u003e\nGene ID: 5925\u003cbr\u003e\nUniProt: View information about Rb on UniProt.org\u003cbr\u003e\nClone: W18116A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: RB Transcriptional Corepressor 1, Retinoblastoma-Associated Protein, Protein Phosphate 1, Regulatory Subunit 130\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866333630633,"sku":"933401","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-933401","provider":"Iright","version":"1.0","type":"link"}