{"product_id":"biolegend-933601","title":"Biolegend, 933601, Purified anti-GLUD1 Antibody, 25μg","description":"\u003cp\u003eGlutamate dehydrogenase (GLUD) is a mitochondrial enzyme involved in the reversible oxidative deamination of glutamate to ammonia and α-ketoglutarate (α-KG), which is a key intermediate in the tricarboxylic acid (TCA) cycle. α-KG is utilized to produce ATP, which in turn increases the ATP\/ADP ratio and induces insulin secretion. GLUD mutations are associated with hyperinsulinism-hyperammonemia (HI\/HA) syndrome. Humans have two isoenzymes, GLUD1 and GLUD2, each encoded by distinct genes. GLUD1 is widely expressed while GLUD2 is expressed in neural and testicular tissues.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant human GLUD1 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality testedICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg\/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone recognizes both human and mouse GLUD1.Methanol permeabilization is recommended for ICC testing. Triton X-100 permeabilization showed weak signal.\u003cbr\u003e\nRRID: AB_2820209 (BioLegend Cat. No. 933601) AB_2820210 (BioLegend Cat. No. 933602)\u003cbr\u003e\nStructure: GLUD1 is a 558 amino acid protein with a predicted molecular weight of 61 kD.\u003cbr\u003e\nDistribution: Mitochondrial matrix\/ubiquitously expressed\u003cbr\u003e\nFunction: Glutaminolysis\u003cbr\u003e\nBiology Area: Cell Biology, Mitochondrial Function\u003cbr\u003e\nMolecular Family: Enzymes and Regulators\u003cbr\u003e\nAntigen References: Stanley CA, et al.1998. N Engl J Med. 338:1352-7 Plaitakis A, et al. 2000. J Neurochem. 75:1862 Yang C, et al. 2009. Cancer Res. 69:7986 Jin L, et al. 2015. Cancer Cell. 27:257\u003cbr\u003e\nGene ID: 2746\u003cbr\u003e\nUniProt: View information about GLUD1 on UniProt.org\u003cbr\u003e\nClone: W18177A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Glutamate Dehydrogenase 1, EC 1.4.1.3, GLUD, Epididymis Tissue Sperm Binding Protein Li 18mP, Epididymis Secretory Sperm Binding Protein, GDH 1\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866335006889,"sku":"933601","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-933601","provider":"Iright","version":"1.0","type":"link"}