{"product_id":"biolegend-933905","title":"Biolegend, 933905, PerCP\/Cyanine5.5 anti-EGFR Antibody, 25tests","description":"\u003cp\u003eEpidermal Growth Factor Receptor (EGFR) is a receptor tyrosine kinase that links extracellular mitogenic ligand binding to complex downstream signaling cascades. Initial ligand binding results in receptor oligomerization and autophosphorylation of multiple tyrosine residues within cytosolic domains of the the protein. These phosphorylation events stabilize the EGFR kinase activation loop and lead to the recruitment of adaptor proteins and other downstream effectors. EGFR stimulation leads to activation of RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCγ-PKC and STAT signaling cascades, and constitutive activation of the receptor promotes tumorigenesis in multiple cancers.\u003cbr\u003e\n25tests\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: Synthetic peptide from human EGFR\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography and conjugated with PerCP\/Cyanine5.5 under optimal conditions.\u003cbr\u003e\nConcentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.\u003cbr\u003e\nApplication: ICFC - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP\/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.\u003cbr\u003e\nExcitation Laser: Blue Laser (488 nm)\u003cbr\u003e\nApplication Notes: For use of this antibody (clone A19002A) in intracellular flow cytometry (ICFC), fixation\/permeabilization using Cyto-Fast™ Perm Buffer (Cat. No. 426803) is recommended.\u003cbr\u003e\nRRID: AB_3662342 (BioLegend Cat. No. 933905) AB_3662342 (BioLegend Cat. No. 933906)\u003cbr\u003e\nStructure: EGFR is a 1,210 amino acid protein with a predicted molecular weight of 134 kD; the processed form is 1,186 amino acids in length.\u003cbr\u003e\nDistribution: Plasma membrane\/Ubiquitously expressed\u003cbr\u003e\nFunction: EGFR signaling, cell signaling\u003cbr\u003e\nCell Type: Endothelial cells, Epithelial cells\u003cbr\u003e\nBiology Area: Cell Biology, Cell Cycle\/DNA Replication, Immunology, Innate Immunity, Neuroscience, Synaptic Biology\u003cbr\u003e\nMolecular Family: CD Molecules, Growth Factors\u003cbr\u003e\nAntigen References: Hunter T and Cooper JA. 1981. Cell. 24:741. Gill GN and Lazar CS. 1981. Nature. 293:305. Reynolds FH, et al. 1981. Nature. 292:259 Zhou M, et al. 2013. Cancer Res. 23:7056-67\u003cbr\u003e\nGene ID: 1956\u003cbr\u003e\nUniProt: View information about EGFR on UniProt.org\u003cbr\u003e\nClone: A19002A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Epidermal Growth Factor Receptor, Receptor Tyrosine-Protein Kinase ErbB-1, Erb-B2 Receptor Tyrosine Kinase 1, ERBB1, NISBD2, PIG61, MENA, Proto-oncogene c-ErbB-1\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003cbr\u003e\nQ: How stable is PerCP\/Cyanine5.5 tandem as compared to PerCP alone?\u003cbr\u003e\nA: PerCP\/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866065129641,"sku":"933905","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-933905","provider":"Iright","version":"1.0","type":"link"}