{"product_id":"biolegend-934302","title":"Biolegend, 934302, Purified anti-TSG101 Antibody, 100μg","description":"\u003cp\u003eTumor Susceptibility Gene 101, TSG101, is a member of the ubiquitin E2 variant (UEV) subfamily of ubiquitin-conjugating enzymes. It is vital to transcriptional regulation, cytokinesis, tissue homeostasis, cell cycle regulation, and cell growth, differentiation, and proliferation. TSG101 is an essential component of the ESCRT-1 (endosomal sorting complexes required for transport) complex, which functions in multivesicular body biogenesis, endosomal trafficking, management of damaged proteins, recycling of membrane receptors, and cellular abscission. Within ESCRT-1, TSG101 binds to ubiquitinated proteins, aids in the formation of multivesicular bodies (MVBs), and recruits subsequent ESCRT components to the complex. Its role as part of ESCRT also makes TSG101 vital to cellular signal transduction, the recycling of receptor tyrosine kinases (RTKs), and Src-mediated migration. TSG101 is essential to the pathogenesis of several viruses - including HIV, influenza, and human papillomavirus - through its facilitation of interactions between the ESCRT complex and viral matrix proteins via the P(T\/S)AP motif, as well as the budding and egress of enveloped viruses. Once predicted to behave as a tumor suppressor due to its role as a negative growth regulator, TSG101’s overexpression in colon, thyroid, breast, ovarian, and lung cancers suggests that TSG101 may actually function as an oncoprotein and play a role in cancer progression.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant TSG101 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested WB- KO\/KD - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg\/mL. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: ICC testing was conducted on HeLa siRNA control cells (positive control) and HeLa TSG101 siRNA knockdown cells (negative control) using three different fix\/perm methods: MeOH fixation, PFA + Triton X-100, and PFA + MeOH. Staining was observed but there was no decrease in signal observed in the siRNA knockdown cells, indicating staining was not specific for TSG101. We therefore do not recommend this clone for the application.\u003cbr\u003e\nRRID: AB_2894522 (BioLegend Cat. No. 934301) AB_2894522 (BioLegend Cat. No. 934302)\u003cbr\u003e\nStructure: TSG101 is a 390 amino acid protein with a predicted molecular weight of 43 kD.\u003cbr\u003e\nDistribution: Ubiquitously expressed\/ nucleus, endosome, cytoplasm, endosome, and cytoskeleton.\u003cbr\u003e\nFunction: Cell cycle, Growth regulation, Host-virus interaction, transcription\u003cbr\u003e\nBiology Area: Cell Biology\u003cbr\u003e\nMolecular Family: Enzymes and Regulators\u003cbr\u003e\nAntigen References: 1. Ferraiuolo R, et al. 2020. Cancers. 12:450. 2. Kumar B et al. 2016. PLOS Pathogens. 12:e1005960. 3. Qadir M and Zafar M. 2017. Crit. Rev. Eukaryot. Gene Expr. 27:237. 4. Tu C, et al. 2011. BioArchitecture. 274:6056. 5. Wagner K, et al. 2003. Mol. Cell. Biol. 1:45.\u003cbr\u003e\nGene ID: 7251\u003cbr\u003e\nUniProt: View information about TSG101 on UniProt.org\u003cbr\u003e\nClone: W19019A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Tumor Susceptibility Gene 101, Tumor Susceptibility Gene 101 Protein, ESCRT-I Complex Subunit TSG101, VPS23 , TSG10\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866341953705,"sku":"934302","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-934302","provider":"Iright","version":"1.0","type":"link"}