{"product_id":"biolegend-938001","title":"Biolegend, 938001, Purified anti-BCAT1 Antibody, 25μg","description":"\u003cp\u003eBCAT1 is an aminotransferase that catalyzes the catabolism of the branched-chain amino acids leucine, isoleucine, and valine, making it an important regulator of the TCA cycle. BCAT1 also plays a role in cell cycle progression, differentiation, and apoptosis. Clinical disorders involved with mutations in the BCAT1 gene include hypervalinemia and hyperleucine-isoleucinemia.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Full-length recombinant human BCAT1 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg\/mL. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone failed to immunoprecipitate BCAT1 from NTERA-2 lysates. This clone was tested for immunocytochemistry on NTERA-2 cells fixed with 4% PFA and permeabilized with either methanol or Triton X-100. Both methods resulted in dim staining of BCAT1. We therefore do not recommend the clone for this application.\u003cbr\u003e\nRRID: AB_2876757 (BioLegend Cat. No. 938001) AB_2876757 (BioLegend Cat. No. 938002)\u003cbr\u003e\nStructure: BCAT1 is a 386 amino acid protein with a predicted molecular weight of 43 kD.\u003cbr\u003e\nDistribution: Ubiquitously expressed\/Cytosolic\u003cbr\u003e\nFunction: Aminotransferase enzyme, amino acid catabolism\u003cbr\u003e\nCell Type: Macrophages\u003cbr\u003e\nBiology Area: Cell Biology, Mitochondrial Function\u003cbr\u003e\nMolecular Family: Enzymes and Regulators\u003cbr\u003e\nAntigen References: Papathanassiu AE, et al. 2017. Nat. Commun. 8:16040. Xu M, et al. 2016. Oncol Lett. 12:2648-2656. Zhou W, et al. 2013. Mol Cancer. 12:53. Wegermann K, et al. 2018. PLoS One. 13:e0204308. Hattori A, et al. 2017. Nature. 545:500-504.\u003cbr\u003e\nGene ID: 586\u003cbr\u003e\nUniProt: View information about BCAT1 on UniProt.org\u003cbr\u003e\nClone: W18024A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Branched Chain Amino Acid Transanimase 1, BCT1, BCATC, PNAS121, Protein ECA39\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866329731241,"sku":"938001","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-938001","provider":"Iright","version":"1.0","type":"link"}