{"product_id":"biolegend-938302","title":"Biolegend, 938302, Purified anti-RAP1 Antibody, 100μg","description":"\u003cp\u003eRepressor\/activator protein 1 (RAP1), also called telomeric repeat-binding factor 2-interacting protein 1 (TERF2IP), is one of six subunits of the shelterin complex, which regulates telomerase activity and protects telomeric DNA through the generation of t-loops. RAP1 forms a heterodimer with shelterin subunit TRF2 and regulates telomere length, recombination, and transcription. This complex also functions in the repression of homology-directed repair (HDR) and prevents telomere deletions. In the cytoplasm, RAP1 functions as an inhibitor kinase (IKK) adaptor and modulator of the nuclear factor- κB (NF-κB) pathway as well as an activator of NF-κB transcriptional activity. TERF2IP S205 phosphorylation has been implicated in the regulation of activation, apoptosis, and senescence of endothelial cells.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant human RAP1 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IP, ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg\/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg\/test. For immunocytochemistry, a concentration of 5.0 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone does not recognize mouse RAP1. ICC testing was conducted on Raji cells fixed with 4% paraformaldehyde and permeabilized with either Triton X-100 or methanol. Both methods were compatible with RAP1 staining.\u003cbr\u003e\nRRID: AB_2876760 (BioLegend Cat. No. 938301) AB_2876760 (BioLegend Cat. No. 938302)\u003cbr\u003e\nStructure: RAP1 is a 399 amino acid protein with an expected molecular weight of 55 kD.\u003cbr\u003e\nDistribution: Ubiquitously expressed\/Nuclear\u003cbr\u003e\nFunction: Transcription, Transcription regulation\u003cbr\u003e\nBiology Area: Cell Biology, DNA Repair\/Replication\u003cbr\u003e\nAntigen References: Cai Y, et al. 2017. Cell Cycle. 16:1765-1773. Kotla S, et al. 2019. JCI Insight. 4(9):124867. Rai R, et al. 2015. Nat Commun. 7:10881. Zhang Y, et al. 2015. Cell Death Discov. 1:15007.\u003cbr\u003e\nGene ID: 54386\u003cbr\u003e\nUniProt: View information about RAP1 on UniProt.org\u003cbr\u003e\nClone: W18011A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: TERF2IP, TERF2 Interacting Protein, TERF2-interacting telomeric protein 1, TRF2-interacting telomeric protein 1, Repressor\/activator protein 1 homolog, Dopamine receptor-interacting protein 5, DRIP5\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866328486057,"sku":"938302","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-938302","provider":"Iright","version":"1.0","type":"link"}