{"product_id":"biolegend-938402","title":"Biolegend, 938402, Purified anti-Vinculin Antibody, 100μg","description":"\u003cp\u003eVinculin is a filamentous actin (F-actin) binding protein that was originally described as a critical component of focal adhesion plaques. It plays an important role in the transmission of mechanical forces, stabilization of cell-cell junctions via a direct interaction with α-catenin, and linking of integrins to the actin cytoskeleton. Loss of Vinculin protein expression contributes to metastatic phenotypes in cancer tissue due to loss of cell-cell adhesion. Vinculin has been isolated from prostate cancer exosomes and may serve as a biomarker for the progression of taxane-resistance prostate cancers; it is also a common marker for cell-cell and focal adhesion junctions.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant human Vinculin protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested ICC, IP, IHC-P - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg\/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg\/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 µg\/test. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg\/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone was tested for ICC using HeLa cells fixed with 4% PFA and permeabilized with Triton X-100 or methanol. While methanol permeabilization was compatible with Vinculin staining, Triton X-100 permeabilization resulted in dim staining. We therefore do not recommend Triton X-100 as a permeabilization method. While this clone passed IP testing, it failed to quantitatively enrich Vinculin from whole cell extracts and may not be suitable for detecting transient or low-abundance protein-protein interactions by co-immunoprecipitation. This clone is predicted to recognize Metavinculin due to complete sequence homology between the immunogen and the corresponding region of Metavinculin.\u003cbr\u003e\nRRID: AB_2876761 (BioLegend Cat. No. 938401) AB_2876761 (BioLegend Cat. No. 938402)\u003cbr\u003e\nStructure: Vinculin is a 1,066 amino acid protein with a predicted molecular weight of 116 kD. Metavinculin is a 1,134 amino acid protein with a predicted molecular weight of 124 kD.\u003cbr\u003e\nDistribution: Ubiquitously expressed\/cytosol, cytoskeleton, and focal adhesion plaques; Metavinculin expression is restricted to smooth skeletal muscle tissue.\u003cbr\u003e\nFunction: Cell-matrix and cell-cell adhesion\u003cbr\u003e\nBiology Area: Cell Biology, Cell Motility\/Cytoskeleton\/Structure\u003cbr\u003e\nMolecular Family: Cytoskeletal Proteins\u003cbr\u003e\nAntigen References: Kawakami K, et al. 2015. Int J Oncol. 47:384-90. Geiger B, et al. 1980. Proc Natl Acad Sci U S A. 77:4127-31. Jockusch BM, et al. 1981. Proc Natl Acad Sci U S A. 78:3005-9.\u003cbr\u003e\nGene ID: 7414\u003cbr\u003e\nUniProt: View information about Vinculin on UniProt.org\u003cbr\u003e\nClone: W18245A\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Metavinculin, MV, Epididymis Secretory Sperm Binding Protein, Epididymis Luminal Protein 114\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866337562793,"sku":"938402","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-938402","provider":"Iright","version":"1.0","type":"link"}