{"product_id":"biolegend-944501","title":"Biolegend, 944501, Purified anti-Htra2\/Omi Antibody, 25μg","description":"\u003cp\u003eHtra2\/Omi (high temperature requirement 2) is a mitochondrial serine protease and member of the high-temperature requirement A family of oligomeric serine proteases that functions in the regulation of apoptosis, mitochondrial homeostasis, and protein quality control. Under normal conditions Htra2\/Omi is localized to the mitochondria where it serves as a chaperone protein. Under stress conditions Htra2\/Omi is released to the cytosol where it mediates apoptosis via proteolytic activity and disruption of caspase-IAP (inhibitors of apoptosis proteins) activity as well as through caspase-independent pathways. Loss or disruption of Htra2\/Omi function results in mitochondrial defects and the aggregation of unfolded or misfolded proteins and has been implicated in various neurodegenerative disorders such as Parkinson’s disease, thus making Htra2\/Omi an important potential therapeutic target in the treatment of such disorders. Likewise, due to its role as a regulator of cell death, Htra2\/Omi may also be an important therapeutic target in the treatment of systemic sepsis and the prevention of Sepsis-Associated Encephalopathy (SAE) as well as a potential oncogenic target and modulator of chemotherapy-induced cytotoxicity.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Mouse\u003cbr\u003e\nImmunogen: recombinant human Htra2 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IP - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg\/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg\/test. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: This clone was tested for ICC on HeLa cells using three different fixation\/permeabilization methods: methanol-only, PFA+ methanol, and PFA+ Triton X-100. Staining was only observed with PFA+ Triton X-100 permeabilization and even this was quite faint and only detected at high exposures. This clone is weakly reactive with mouse Htra2 by western blot.\u003cbr\u003e\nRRID: AB_2890873 (BioLegend Cat. No. 944501) AB_2890873 (BioLegend Cat. No. 944502)\u003cbr\u003e\nStructure: HTRA2 is a 458 amino acid protein with an expected molecular weight of 38 to 49 kD.\u003cbr\u003e\nDistribution: Ubiquitous\/ Mitochondria\u003cbr\u003e\nFunction: Protease, Hydrolase, Apoptosis\u003cbr\u003e\nAntigen References: 1. Chen S, et al. 2018. Genes. 9:355. 2. Chien J, et al. 2009. Curr. Cancer Drug Targets. 9:451. 3. Hu Y, et al. 2019. Front. Mol. Neurosci. 12:48. 4. Zurawa-Janicka D, et al. 2013. Cell Stress Chaperones. 18:35.\u003cbr\u003e\nGene ID: 27429\u003cbr\u003e\nUniProt: View information about Htra2\/Omi on UniProt.org\u003cbr\u003e\nClone: 8G11-4B8-4F10-G5\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: HTRA Serine Petidase 2, OMI, PRSS25, PARK13, High Temperature Requirement Protein A2, Serine Protease HTRA2 Mitochondrial, OMI Stress-Regulated Endoprotease, Serine Proteinase OMI, MGCA8\u003cbr\u003e\nIsotype: Mouse IgG1, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46862496399529,"sku":"944501","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-944501","provider":"Iright","version":"1.0","type":"link"}