{"product_id":"biolegend-945102","title":"Biolegend, 945102, Purified anti-SARS-CoV-2 S Protein S1 Antibody, 100μg","description":"\u003cp\u003eSARS-CoV-2 is a respiratory virus which causes coronavirus disease 2019 (COVID-19). The coronavirus spike (S) glycoprotein is a class I viral fusion protein on the outer envelope of the virion that plays a critical role in viral infection by recognizing host cell receptors and mediating fusion of the viral and cellular membranes. The S glycoprotein is synthesized as a precursor protein consisting of ~1,300 amino acids that is then cleaved into an amino (N)-terminal S1 subunit (~700 amino acids) and a carboxyl (C)-terminal S2 subunit (~600 amino acids). Three S1\/S2 heterodimers assemble to form a trimer spike protruding from the viral envelope. The S1 subunit contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. Triggered by receptor binding, proteolytic processing and\/or acidic pH in the cellular compartments, the class I viral fusion protein undergoes a transition from a metastable pre-fusion state to a stable post-fusion state during infection, in which the receptor-binding subunit is cleaved, and the fusion subunit undergoes large-scale conformational rearrangements to expose the hydrophobic fusion peptide, induce the formation of a six-helix bundle, and bring the viral and cellular membranes close for fusion. The trimeric SARS coronavirus (SARS-CoV-2) S glycoprotein consisting of three S1-S2 heterodimers binds the cellular receptor angiotensin-converting enzyme 2 (ACE2) and mediates fusion of the viral and cellular membranes through a pre- to post-fusion conformation transition.\u003cbr\u003e\n100μg\u003cbr\u003e\nVerified Reactivity: SARS-CoV-2\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant SARS-CoV-2 S protein corresponding to S1 subunit\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested Direct ELISA, FC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1.0 µg\/mL. For Direct ELISA, a concentration of 114.9 ng\/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: BioLegend offers multiple clones that recognize SARS-CoV-2 S protein S1. Clone A20103H displayed the strongest performance for western blot of all clones validated for the application.\u003cbr\u003e\nProduct Citations: Mamedov T, et al. 2021. Viruses. 13:. PubMed\u003cbr\u003e\nRRID: AB_2890875 (BioLegend Cat. No. 945101) AB_2890875 (BioLegend Cat. No. 945102)\u003cbr\u003e\nStructure: Spike glycoprotein is a homotrimer. Each monomer consists of 1,273 amino acids with a theoretical molecular weight of 141 kD, and consists of S1 and S2 subunits.\u003cbr\u003e\nDistribution: Viral envelope protein, host cell membrane, host cell endoplasmic reticulum-Golgi intermediate compartment membrane\u003cbr\u003e\nFunction: Mediates fusion between virus and host cell membranes\u003cbr\u003e\nInteraction: ACE2\u003cbr\u003e\nLigand\/Receptor: ACE2\u003cbr\u003e\nBiology Area: COVID-19\u003cbr\u003e\nAntigen References: 1. Walls AC, et al. 2020. Cell. 181(2):281-292. 2. Yan R, et al. 2020. Science. 367 (6485):1444-1448. 3. Wrapp D, et al. 2020. Science. 367 (6483):1260-1263. 4. Shang J, et al. 2020. PNAS. 117(21):11727-11734\u003cbr\u003e\nGene ID: 43740568\u003cbr\u003e\nUniProt: View information about Viral Protein on UniProt.org\u003cbr\u003e\nClone: A20103O\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: S1, Spike Protein\u003cbr\u003e\nIsotype: Rat IgG2a, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866071191721,"sku":"945102","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-945102","provider":"Iright","version":"1.0","type":"link"}