{"product_id":"biolegend-947501","title":"Biolegend, 947501, Purified anti-ERCC1 Antibody, 25μg","description":"\u003cp\u003eERCC1, also referred to as Excision Repair Cross-Complementation Group 1, is a component of the nucleotide excision repair pathway. ERCC1 forms a heterodimer with XPF endonuclease to catalyze the 5’ incision in the process of excising a DNA lesion. ERCC1 also works with SLX4 in homology direct repair of double strand breaks and in the repair of crosslinks. Mutations in ERCC1 play a role in carcinogenesis due to genome instability, and elevated expression of ERCC1 is predictive of resistance to platinum-based chemotherapies in multiple types of cancers.\u003cbr\u003e\n25μg\u003cbr\u003e\nVerified Reactivity: Human, Mouse\u003cbr\u003e\nAntibody Type: Monoclonal\u003cbr\u003e\nHost Species: Rat\u003cbr\u003e\nImmunogen: Partial recombinant human ERCC1 protein\u003cbr\u003e\nFormulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide\u003cbr\u003e\nPreparation: The antibody was purified by affinity chromatography.\u003cbr\u003e\nConcentration: 0.5 mg\/mL\u003cbr\u003e\nStorage \u0026amp; Handling: The antibody solution should be stored undiluted between 2°C and 8°C.\u003cbr\u003e\nApplication: WB - Quality tested IHC-P, ICC - Verified\u003cbr\u003e\nRecommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg\/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg\/mL is suggested. For immunocytochemistry, a concentration range of 0.625 - 5.0 μg\/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.\u003cbr\u003e\nApplication Notes: Clone W20045D detects multiple ERCC1 isoforms by western blot. Clone W20045D was tested for ICC using HeLa cells fixed with methanol or 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. All three methods were compatible with ERCC1 staining.\u003cbr\u003e\nRRID: AB_2894534 (BioLegend Cat. No. 947501) AB_2894534 (BioLegend Cat. No. 947502)\u003cbr\u003e\nStructure: ERCC1 is a 297 amino acid protein with an expected molecular weight of 33 kD. Four isoforms between 25 and 36 kD have been described.\u003cbr\u003e\nDistribution: Ubiquitously expressed\/ Nucleus\u003cbr\u003e\nFunction: DNA damage repair\u003cbr\u003e\nBiology Area: Cell Biology, DNA Repair\/Replication\u003cbr\u003e\nMolecular Family: Enzymes and Regulators\u003cbr\u003e\nAntigen References: Friboulet, et al. 2013. Cell Cycle. 12:3298 Kashiyama, et al. 2013. J Hum Genet. 92:807 Wan J, et al. 2017. Canc Invest. 35:85 Faridounnia, et al. 2018. Molecules. 23:3205\u003cbr\u003e\nGene ID: 2067\u003cbr\u003e\nUniProt: View information about ERCC1 on UniProt.org\u003cbr\u003e\nClone: W20045D\u003cbr\u003e\nRegulatory Status: RUO\u003cbr\u003e\nOther Names: Excision Repair Cross-Complementation Group 1, ERCC excision repair 1, endonuclease non-catalytic subunit, RAD10\u003cbr\u003e\nIsotype: Rat IgG2b, κ\u003c\/p\u003e","brand":"Biolegend","offers":[{"title":"Default Title","offer_id":46866418630825,"sku":"947501","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/biolegend-947501","provider":"Iright","version":"1.0","type":"link"}