{"product_id":"cst-23765sf","title":"CST,  23765SF, VSV-G Tag (E8S5G) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying . Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, VSV-G Tag (E8S5G) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #23765) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #93372. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 93372\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nVSV-G Tag (E8S5G) Rabbit Monoclonal Antibody (BSA and Azide Free) detects recombinant proteins containing the VSV-G epitope tag. This antibody recognizes the VSV-G tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells. This antibody detects exogenously expressed VSV-G tagged proteins in cells expressed under a CMV promoter. Expression under other promoters has not been evaluated.\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide containing the vesicular stomatitis virusÂ glycoprotein epitope (YTDIEMNRLGK).\n\u003cb\u003eBackground\u003c\/b\u003e\nEpitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The VSV-G tag is derived from an epitope from the C-terminal region ofÂ vesicular stomatitis virus glycoprotein (1).Â VSV-G is widely used both as an epitope tag in expression vectors and for pseudotyping lentiviral vectors due to the stability and broad host range of the resulting pseudotypes (2,3).\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Transfected Only\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800206463145,"sku":"23765SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/cst-23765sf","provider":"Iright","version":"1.0","type":"link"}