{"product_id":"cst-3410s","title":"CST,  3410S, Drosha (D30F3) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Drosha. Validated for Western Blotting,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, Drosha (D30F3) Rabbit Monoclonal Antibody (CST #3410) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 20 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nChromatin IP: 1:25\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nDrosha (D30F3) Rabbit Monoclonal Antibody detects endogenous levels of total Drosha protein.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His549 of human Drosha.\n\u003cb\u003eBackground\u003c\/b\u003e\nDrosha was identified as a nuclear RNase III that catalyzes the initial step of microRNA (miRNA) processing (1). This enzyme processes the long primary transcript pri-miRNAs into stem-looped pre-miRNAs. Interference of Drosha results in the increase of pri-miRNAs and the decrease of pre-miRNAs (1). Drosha exists in a multiprotein complex called Microprocessor along with other components, such as DGCR8 (2). Drosha and DGCR8 are necessary for miRNA biogenesis (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDrosha; drosha ribonuclease III; drosha, double-stranded RNA-specific endoribonuclease; drosha, ribonuclease type III; ETOHI2; HSA242976; nuclear RNase III Drosha; p241; Protein Drosha; putative protein p241 which interacts with transcription factor Sp1; putative ribonuclease III; RANSE3L; Ribonuclease 3; Ribonuclease III; ribonuclease III, nuclear; ribonuclease type III, nuclear; RN3; RNase III; RNASE3L; RNASEN; RNC\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 160\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799416033449,"sku":"3410S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/cst-3410s","provider":"Iright","version":"1.0","type":"link"}