{"product_id":"cst-35016s","title":"CST,  35016S, Whitlow\/218 Linker (1B4A1) Mouse Monoclonal Antibody (Biotinylated)","description":"Monoclonal Antibody for studying . Validated for Flow Cytometry (Fixed\/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, Whitlow\/218 Linker (1B4A1) Mouse Monoclonal Antibody (Biotinylated) (CST #35016) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFlow Cytometry (Fixed\/Permeabilized): 1:50\nFlow Cytometry (Live): 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Fixed\/Permeabilized), Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nWhitlow\/218 Linker (1B4A1) Mouse Monoclonal Antibody (Biotinylated) recognizes exogenously expressed levels of scFv-based CARs containing a Whitlow\/218 linker.\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues of the Whitlow\/218 linker peptide.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe Whitlow\/218 peptide is a commonly used linker that connects the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The Whitlow\/218 linker affords the scFv with enhanced resistance to proteolysis and aggregation (1).\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Transfected Only\nSource\/Isotype: Mouse IgG2a","brand":"CST","offers":[{"title":"Default Title","offer_id":46800383934633,"sku":"35016S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/cst-35016s","provider":"Iright","version":"1.0","type":"link"}