{"product_id":"cst-35986s","title":"CST,  35986S, NK1.1\/CD161 (PK136) Mouse Monoclonal Antibody (APC Conjugate)","description":"Monoclonal Antibody for studying KLRB1C mouse. Validated for Flow Cytometry (Fixed\/Permeabilized),Flow Cytometry (Live). Highly specific and rigorously validated in-house, NK1.1\/CD161 (PK136) Mouse Monoclonal Antibody (APC Conjugate) (CST #35986) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal flow cytometry results, we recommend 0.125 μg of antibody per test.\nFlow Cytometry (Fixed\/Permeabilized): 1:160\nFlow Cytometry (Live): 1:160\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Fixed\/Permeabilized), Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNK1.1\/CD161 (PK136) Mouse Monoclonal Antibody (APC Conjugate) recognizes endogenous levels of total NK1.1\/CD161 protein. This antibody conjugate detects an epitope within the extracellular domain.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nThis monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.\n\u003cb\u003eBackground\u003c\/b\u003e\nCD161\/KLRB1 (Killer cell lectin-like receptor subfamily B member 1, also known as CLEC5B and NKR-P1A) is a type II transmembrane protein that is expressed on the majority of Natural Killer (NK) cells, NK T cells, and some T lymphocytes (1). CD161\/KLRB1 is also expressed on Th17 cells, promotes their generation, and modulates their function (2). Engagement with its ligand lectin-like transcript 1 (LLT1) inhibits NK cell function, while LLT1 and CD161\/KLRB1 interaction in the presence of a TCR signal enhances IFN-gamma production by T cells (3,4). There are several different CD161 isoforms in rodents and some function as activating receptors as well (5,6). There is a family of Klrb1 genes in rodents (7). PK136 antibody recognizes a specific epitope on mouse KLRB1b\/c, also referred to as NK1.1, and is commonly used for detection of mouse NK cells in certain mouse strains (CE, B6, NZB, C58, Ma\/My, ST, SJL, FVB). However, the epitope is absent in other mouse strains (BALB\/c, AKR, CBA, C3H, DBA, 129) (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAI462337; CD161; CD161 antigen-like family member C; CD161c; killer cell lectin-like receptor subfamily A member 1C; killer cell lectin-like receptor subfamily B member 1C; Killer cell lectin-like receptor subfamily B member 1C; Klrb1b; Klrb1c; KLRBC; Ly-5; Ly-55c; Ly-59; Ly5; Ly55; Ly55c; Ly59; lymphocyte antigen 55 complex, locus C; Lymphocyte antigen 55c; lymphocyte antigen 59; natural killer cell receptor protein NKR-P1C; natural killer cell receptor-P1; Natural killer cell surface protein P1-40; natural killer cell-associated antigen 1; NK-; Nk-1; NK-1.; Nk-1.2; NK-RP1; Nk1; Nk1.; NK1.1; Nk1.2; NKR; NKR-; NKR-P1 40; NKR-P1.9; NKR-P1B; NKR-P1C; Nkrp; NKRP1; NKRP140; Nkrp1b; Nkrp1c\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nSource\/Isotype: Mouse IgG2a kappa","brand":"CST","offers":[{"title":"Default Title","offer_id":46800385769641,"sku":"35986S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/cst-35986s","provider":"Iright","version":"1.0","type":"link"}