{"product_id":"cst-4045s","title":"CST,  4045S, WWOX Antibody","description":"Polyclonal Antibody for studying WWOX. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, WWOX Antibody (CST #4045) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nWWOX Antibody detects endogenous levels of total WWOX protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr103 of WWOX. Antibodies were purified by peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe (WW domain-containing oxidoreductase) gene encodes a protein with two WW domains followed by a short-chain dehydrogenase domain that was identified from a genomic region 16q23 of high instability, FRA16D (1,2). The mouse homolog, termed Wox1, was found to enhance TNFα-mediated apoptosis (3). The gene is disrupted in a many cancer types by deletions or translocation which has revealed a tumor suppressor function (4-7). In contrast, high levels of WWOX have been shown in shown in premetastic cancers, including breast and prostate (8-10). Stress stimuli can induce tyrosine phosphorylation within the first WW domain (Tyr33), followed by nuclear translocation and binding to and stabilizing the p53 tumor suppressor protein (11). WWOX and p53 can induce apoptosis in a synergistic manner. Tyrosine phosphorylation and nuclear translocation of WWOX has been implicated in the progression of cancers to metastatic states (10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nD16S432E; EIEE28; FOR; FRA16D; Fragile site FRA16D oxidoreductase; HHCMA56; PRO0128; SCAR12; SDR41C1; Short chain dehydrogenase\/reductase family 41C member 1; short chain dehydrogenase\/reductase family 41C, member 1; WOX1; WW domain containing oxidoreductase; WW domain-containing oxidoreductase; WW domain-containing protein WWOX; WWOX\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 46\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799478489257,"sku":"4045S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/cst-4045s","provider":"Iright","version":"1.0","type":"link"}