{"product_id":"cst-4462s","title":"CST,  4462S, Ran Antibody","description":"Polyclonal Antibody for studying Ran. Validated for Western Blotting. Highly specific and rigorously validated in-house, Ran Antibody (CST #4462) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nRan Antibody detects endogenous levels of total Ran protein. This antibody does not cross-react with other small GTPases of the Ras family.\nSpecies Reactivity: Human, Mouse, Rat, Hamster, Monkey, Bovine\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with two synthetic peptides corresponding to amino acids surrounding Arg29 and Asn143 of human Ran. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nRan is a small GTPase of the Ras family that plays a central role in the spacial and temperal organization of eukaryotic cells. During interphase, Ran-GDP localizes to the cytoplasm and Ran-GTP to the nucleus. This polarized localization of Ran ensures its role in nuclear transport (1). During mitosis, Ran-GTP is chromatin associated, where it promotes spindle assembly and nuclear envolope formation (1,2). In S phase, Ran-GTP associates with and inhibits MCM helicase, ensuring precise chromosomal DNA duplication during the cell cycle (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAndrogen receptor-associated protein 24; ARA24; Gsp1; GTP-binding nuclear protein Ran; GTPase Ran; guanosine triphosphatase Ran; member RAS oncogene family; OK\/SW-cl.81; RAN; RAN, member RAS oncogene family; RanGTPase; Ras-like protein TC4; Ras-related nuclear protein; TC4\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Hm Mk B\nSENSITIVITY: Endogenous\nMW (kDa): 24\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46799513845929,"sku":"4462S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/cst-4462s","provider":"Iright","version":"1.0","type":"link"}