{"product_id":"cst-44665t","title":"CST,  44665T, XCR1 (D2F8T) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying XCR1. Validated for Western Blotting,IHC Leica Bond,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, XCR1 (D2F8T) Rabbit Monoclonal Antibody (CST #44665) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nIHC Leica Bond: 1:50 - 1:200\nImmunohistochemistry (Paraffin): 1:100 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 63684 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nXCR1 (D2F8T) Rabbit Monoclonal Antibody recognizes endogenous levels of total XCR1 protein by IHC-P and transfected levels of XCR1 protein by Western. Staining of unknown specificity in peripheral nerves has been observed.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human XCR1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nX-C motif chemokine receptor 1 (XCR1, GPR5, CCXCR1), part of the G protein-coupled superfamily, is expressed by a subset of dendritic cells and acts as a receptor for chemokines XCL1 and XCL2 (1,2,4). XCR1-positive dendritic cells cross-present antigens to naïve CD8 T cells, priming them to become activated cytotoxic CD8 T cells (3-5). In mouse models, the XCL1-XCR1 signaling axis was shown to be involved in the formation of self-tolerance through the development of T cells within the thymus (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCCXCR1; chemokine (C motif) receptor 1; chemokine (C motif) XC receptor 1; Chemokine XC receptor 1; G protein-coupled receptor 5; G-protein coupled receptor 5; GPR5; Lymphotactin receptor; X-C motif chemokine receptor 1; XC chemokine receptor 1; XCR1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous (IHC-P), Transfected (W)\nMW (kDa): 35\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46799514566825,"sku":"44665T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/cst-44665t","provider":"Iright","version":"1.0","type":"link"}