{"product_id":"cst-83932s","title":"CST,  83932S, Oct-4A (D6C8T) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Oct4A mouse. Validated for WB,IP,IF,F,ChIP,C\u0026amp;R. Available in 2 sizes. Highly specific and rigorously validated in-house, Oct-4A (D6C8T) Rabbit Monoclonal Antibody (CST #83932) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. The CUT\u0026amp;RUN dilution was determined using CUT\u0026amp;RUN Assay Kit # 86652 .\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunofluorescence (Immunocytochemistry): 1:800\nFlow Cytometry (Fixed\/Permeabilized): 1:200\nChromatin IP: 1:50\nCUT\u0026amp;RUN: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized), Chromatin IP, CUT\u0026amp;RUN\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nOct-4A (D6C8T) Rabbit Monoclonal Antibody recognizes endogenous levels of total murine Oct-4A protein.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val80 of murine Oct-4A protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nOct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells (6,7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nNF-A3; Oct; Oct-; Oct-3; Oct-3\/; Oct-3\/4; Oct-4; Oct3; Oct3\/; Oct3\/4; Oct4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; octamer-binding transcription factor 4 alternative; Otf; Otf-; OTF-3; Otf-4; Otf3; Otf3-; Otf3-rs7; Otf3g; Otf4; PO5F1; POU domain class 5 transcription factor 1 transcript variant 2 oct4b'; POU domain class 5 transcription factor 1 transcript variant 3 oct4b; POU domain, class 5, transcription factor 1; Pou5f1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 45\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800132997289,"sku":"83932S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/cst-83932s","provider":"Iright","version":"1.0","type":"link"}