{"product_id":"proteintech-10142-2-ap","title":"Proteintech, 10142-2-AP, PRKAR2A Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PRKAR2A (10142-2-AP) by Proteintech is a Polyclonal antibody targeting PRKAR2A in WB, IHC, IF-P, IP, ELISA applications with reactivity to human, mouse, rat samples\n10142-2-AP targets PRKAR2A in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, K-562 cells,  MCF-7 cells,  mouse testis tissue,  PC-3 cells,  human testis tissue\nPositive IP detected in: HeLa cells, HeLa  cells\nPositive IHC detected in: mouse colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse testis tissue\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory and two catalytic subunits. The regulatory subunit  of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2.  The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy. This antibody got two bands about 45 kDa and 50-55 kDa in western blotting, and the 50-55kDa may be caused by phosphorylation.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag0203 Product name: Recombinant human PRKAR2A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 74-382 aa of BC002763 Sequence: AKGDSESEEDEDLEVPVPSRFNRRVSVCAETYNPDEEEEDTDPRVIHPKTDEQRCRLQEACKDILLFKNLDQEQLSQVLDAMFERIVKADEHVIDQGDDGDNFYVIERGTYDILVTKDNQTRSVGQYDNRGSFGELALMYNTPRAATIVATSEGSLWGLDRVTFRRIIVKNNAKKRKMFESFIESVPLLKSLEVSERMKIVDVIGEKIYKDGERIITQTKSNKDGGNQEVEIARCHKGQYFGELALVTNKPRAASAYAVGDVKCLVMDVQAFERLLGPCMDIMKRNISHYEEQLVKMFGSSVDLGNLGQ Predict reactive species\nFull Name: protein kinase, cAMP-dependent, regulatory, type II, alpha\nCalculated Molecular Weight: 43 kDa\nObserved Molecular Weight: 46-52 kDa\nGenBank Accession Number: BC002763\nGene Symbol: PRKAR2A\nGene ID (NCBI): 5576\nRRID: AB_2168236\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P13861\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868987052201,"sku":"10142-2-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-10142-2-ap","provider":"Iright","version":"1.0","type":"link"}