{"product_id":"proteintech-10219-1-ap","title":"Proteintech, 10219-1-AP, EIF3D Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe EIF3D (10219-1-AP) by Proteintech is a Polyclonal antibody targeting EIF3D in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, yeast samples\n10219-1-AP targets EIF3D in WB, IHC, IF\/ICC, IP, CoIP, ELISA, PLA applications and shows reactivity with human, mouse, yeast samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse brain tissue, A549 cells,  HepG2 cells,  human brain tissue,  L02 cells,  HEK-293 cells,  Jurkat cells\nPositive IP detected in: A549 cells\nPositive IHC detected in: human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells, Ethacrynic acid treated HepG2 cells,  transfected cells,  N\/A,  MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, yeast\nCited Reactivity: human, mouse, rat, rabbit, yeast\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag0268 Product name: Recombinant human EIF3D protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 323-531 aa of BC000328 Sequence: FSQQCLRMGKERYNFPNPNPFVEDDMDKNEIASVAYRYRRWKLGDDIDLIVRCEHDGVMTGANGEVSFINIKTLNEWDSRHCNGVDWRQKLDSQRGAVIATELKNNSYKLARWTCCALLAGSEYLKLGYVSRYHVKDSSRHVILGTQQFKPNEFASQINLSVENAWGILRCVIDICMKLEEGKYLILKDPNKQVIRVYSLPDGTFSSDE Predict reactive species\nFull Name: eukaryotic translation initiation factor 3, subunit D\nCalculated Molecular Weight: 66 kDa\nObserved Molecular Weight: 66 kDa\nGenBank Accession Number: BC000328\nGene Symbol: EIF3D\nGene ID (NCBI): 8664\nRRID: AB_2096880\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O15371\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868865843369,"sku":"10219-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-10219-1-ap","provider":"Iright","version":"1.0","type":"link"}