{"product_id":"proteintech-10355-1-ap","title":"Proteintech, 10355-1-AP, P21 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe P21 (10355-1-AP) by Proteintech is a Polyclonal antibody targeting P21 in WB, IHC, IF\/ICC, IF-P, FC (Intra), IP, ELISA applications with reactivity to human samples\n10355-1-AP targets P21 in WB, IHC, IF\/ICC, IF-P, FC (Intra), IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HUVEC cells, MCF-7 cells,  HeLa cells,  HEK-293 cells,  HepG2 cells,  THP-1 cells,  LNCaP cells,  NCCIT cells,  U2OS cells\nPositive IP detected in: MCF-7 cells\nPositive IHC detected in: human colon cancer tissue, human breast cancer tissue,  human cervical cancer tissue,  human kidney tissue,  human stomach cancer tissue,  human thyroid cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: myotome of a somite at E11.5 tissue\nPositive IF\/ICC detected in: MCF-7 cells\nPositive FC (Intra) detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:5000-1:20000\nImmunofluorescence (IF)-P: IF-P : 1:10-1:100\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, pig, rabbit, canine, bovine, artemia sinica\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag0368 Product name: Recombinant human P21;CDKN1A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-149 aa of BC000275 Sequence: MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTD Predict reactive species\nFull Name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)\nCalculated Molecular Weight: 18 kDa\nObserved Molecular Weight: 21 kDa\nGenBank Accession Number: BC000275\nGene Symbol: p21\nGene ID (NCBI): 1026\nRRID: AB_2077682\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P38936\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868817707177,"sku":"10355-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-10355-1-ap","provider":"Iright","version":"1.0","type":"link"}