{"product_id":"proteintech-10384-1-ap","title":"Proteintech, 10384-1-AP, Cytokeratin 8 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe Cytokeratin 8 (10384-1-AP) by Proteintech is a Polyclonal antibody targeting Cytokeratin 8 in WB, IHC, IF\/ICC, IF-P, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples\n10384-1-AP targets Cytokeratin 8 in WB, IHC, IF\/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, HaCaT cells,  COLO 320 cells,  A431 cells,  A549 cells,  MCF-7 cells,  mouse skin tissue,  rat skin tissue\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human tonsillitis tissue, human appendicitis tissue,  human bowen disease tissue,  human breast cancer tissue,  human colon tissue,  human liver tissue,  human renal cell carcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: human breast cancer tissue\nPositive IF\/ICC detected in: MCF-7 cells, HeLa cells,  A431 cells\nPositive FC (Intra) detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:40000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:3000-1:12000\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCytokeratin 8 (CK8) is a protein that belongs to the cytokeratin family, which is a group of structural proteins found in the intracytoplasmic cytoskeleton of epithelial cells. CK8 forms intermediate filaments within the cytoplasm of simple epithelial cells, typically as a dimer with cytokeratin 18 (CK18). These filaments provide a stabilizing framework that helps determine cell shape and enables cells to cope with mechanical stress. CK8\/CK18 filaments also serve as a mesh of \"paths\" for signaling molecules, metabolites, and pathogens to travel within the cell in an organized manner. CK8 is expressed in various epithelial tissues and is particularly associated with glandular and simple epithelia. Its expression is also a significant marker in several types of cancer, including lung and esophageal cancer, where it can indicate tumor progression and poor prognosis.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, pig, canine, goat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag0488 Product name: Recombinant human KRT8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-483 aa of BC000654 Sequence: MSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSSNFRGGLGGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLKLEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQLYEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRAEAESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAAIADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSSSFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK Predict reactive species\nFull Name: keratin 8\nCalculated Molecular Weight: 54 kDa\nObserved Molecular Weight: 52 kDa\nGenBank Accession Number: BC000654\nGene Symbol: Cytokeratin 8\nGene ID (NCBI): 3856\nRRID: AB_10638912\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P05787\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868924104873,"sku":"10384-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-10384-1-ap","provider":"Iright","version":"1.0","type":"link"}