{"product_id":"proteintech-10499-1-ap","title":"Proteintech, 10499-1-AP, TFII I Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe TFII I (10499-1-AP) by Proteintech is a Polyclonal antibody targeting TFII I in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n10499-1-AP targets TFII I in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, HeLa cells,  Jurkat cells\nPositive IHC detected in: human thyroid cancer tissue, human colon cancer tissue,  human urothelial carcinoma tissue,  mouse small intestine tissue,  rat kidney tissue,  rat small intestine tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe GTF2I gene encodes a transcription factor. It negatively regulates agonist-induced calcium entry into cells by interfering with expression of the cation channel TRPC3 at the plasma membrane [PMID: 17023658]. As a transcription factor and\/or as a regulator of intracellular calcium levels, GTF2I may play a role in the molecular basis of anxiety[PMID: 22578324]. It interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation [PMID: 16738337].\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag0773 Product name: Recombinant human GTF2I protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-274 aa of BC004472 Sequence: MAQVAMSTLPVEDEESSESRMVVTFLMSALESMCKELAKSKAEVACIAVYETDVFVVGTERGRAFVNTRKDFQKDFVKYCVEEEEKAAEMHKMKSTTQANRMSVDAVEIETLRKTVEDYFCFCYGKALGKSTVVPVPYEKMLRDQSAVVVQGLPEGVAFKHPENYDLATLKWILENKAGISFIIKRPFLEPKKHVGGRVMVTDADRSILSPGGSCGPIKVKTEPTEDSGISLEMAAVTVKEESEDPDYYQYNIQGSHHSSEGNEGTEMEVPAEG Predict reactive species\nFull Name: general transcription factor II, i\nCalculated Molecular Weight: 112 kDa\nObserved Molecular Weight: 135 kDa\nGenBank Accession Number: BC004472\nGene Symbol: TFII I\nGene ID (NCBI): 2969\nRRID: AB_2114813\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P78347\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869503770793,"sku":"10499-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-10499-1-ap","provider":"Iright","version":"1.0","type":"link"}