{"product_id":"proteintech-10584-1-ap","title":"Proteintech, 10584-1-AP, IA-2\/PTPRN Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe IA-2\/PTPRN (10584-1-AP) by Proteintech is a Polyclonal antibody targeting IA-2\/PTPRN in WB, IHC, IF-P, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples\n10584-1-AP targets IA-2\/PTPRN in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: mouse brain tissue,  HEK-293 cells,  mouse kidney tissue,  rat brain tissue\nPositive IP detected in: mouse brain tissue\nPositive IHC detected in: rat pancreas tissue, human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse pancreas tissue\nPositive FC (Intra) detected in: HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nIA-2\/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2\/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell).(PMID: 10027571). IA-2\/PTPRN was found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2\/PTPRN is commonly used as a diabetes diagnosis marker.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag0923 Product name: Recombinant human PTPRN protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 510-811 aa of BC007713 Sequence: WCEEPAQANMDISTGHMILAYMEDHLRNRDRLAKEWQALCAYQAEPNTCATAQGEGNIKKNRHPDFLPYDHARIKLKVESSPSRSDYINASPIIEHDPRMPAYIATQGPLSHTIADFWQMVWESGCTVIVMLTPLVEDGVKQCDRYWPDEGASLYHVYEVNLVSEHIWCEDFLVRSFYLKNVQTQETRTLTQFHFLSWPAEGTPASTRPLLDFRRKVNKCYRGRSCPIIVHCSDGAGRTGTYILIDMVLNRMAKGVKEIDIAATLEHVRDQRPGLVRSKDQFEFALTAVAEEVNAILKALPQ Predict reactive species\nFull Name: protein tyrosine phosphatase, receptor type, N\nCalculated Molecular Weight: 105 kDa\nObserved Molecular Weight: 60-65 kDa, 106-115 kDa\nGenBank Accession Number: BC007713\nGene Symbol: IA-2\/PTPRN\nGene ID (NCBI): 5798\nRRID: AB_2237983\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q16849\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868982857897,"sku":"10584-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-10584-1-ap","provider":"Iright","version":"1.0","type":"link"}