{"product_id":"proteintech-10736-1-ap","title":"Proteintech, 10736-1-AP, VCP Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe VCP (10736-1-AP) by Proteintech is a Polyclonal antibody targeting VCP in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples\n10736-1-AP targets VCP in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, human brain tissue,  rat lung tissue,  mouse lung tissue,  U2OS cells,  C6 cells,  mouse thymus tissue,  mouse brain tissue,  rat brain tissue,  SH-SY5Y cells,  NIH\/3T3 cells\nPositive IP detected in: HeLa cells, mouse brain tissue\nPositive IHC detected in: human gliomas tissue,  human colon cancer tissue,  human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:16000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:100-1:400\n\u003cb\u003eBackground Information\u003c\/b\u003e\nVCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, pig\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1002 Product name: Recombinant human VCP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 345-644 aa of BC007562 Sequence: GMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG Predict reactive species\nFull Name: valosin-containing protein\nCalculated Molecular Weight: 89 kDa\nObserved Molecular Weight: 90-100 kDa\nGenBank Accession Number: BC007562\nGene Symbol: VCP\nGene ID (NCBI): 7415\nRRID: AB_2214635\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P55072\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868841365673,"sku":"10736-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-10736-1-ap","provider":"Iright","version":"1.0","type":"link"}