{"product_id":"proteintech-10793-1-ap","title":"Proteintech, 10793-1-AP, REC8 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nREC8 Polyclonal Antibody for WB, ELISA\n10793-1-AP targets REC8 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: human testis tissue,  K-562 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nREC8, also named as Rec8p and REC8L1, belongs to the rad21 family. This cohesin protein is required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II. Rec8 is present in an unphosphorylated form early in meiotic prophase but is phosphorylated prior to meiosis I. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human REC8. This antibody can recognize  the monomeric, non-phosphorylated form(63-70kd) of REC8 and other forms(80-100kd) which represent either posttranslationally modified forms or alternative transcript products (PMID: 12615909,19463812).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1250 Product name: Recombinant human REC8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 188-548 aa of BC010887 Sequence: VLPPEAITILEAEPIRMLEIEGERELPEVSRRELDLLIAEEEEAILLEIPRLPPPAPAEVEGIGEALGPEELRLTGWEPGALLMEVTPPEELRLPAPPSPERRPPVPPPPRRRRRRRLLFWDKETQISPEKFQEQLQTRAHCWECPMVQPPERTIRGPAELFRTPTLSGWLPPELLGLWTHCAQPPPKALRRELPEEAAAEEERRKIEVPSEIEVPREALEPSVPLMVSLEISLEAAEEEKSRISLIPPEERWAWPEVEAPEAPALPVVPELPEVPMEMPLVLPPELELLSLEAVHRAVALELQANREPDFSSLVSPLSPRRMAARVFYLLLVLSAQQILHVKQEKPYGRLLIQPGPRFH Predict reactive species\nFull Name: REC8 homolog (yeast)\nCalculated Molecular Weight: 63 kDa\nObserved Molecular Weight: 85-95 kDa\nGenBank Accession Number: BC010887\nGene Symbol: REC8\nGene ID (NCBI): 9985\nRRID: AB_2178282\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O95072\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868908081321,"sku":"10793-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-10793-1-ap","provider":"Iright","version":"1.0","type":"link"}