{"product_id":"proteintech-10828-1-ap","title":"Proteintech, 10828-1-AP, c-MYC Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe c-MYC (10828-1-AP) by Proteintech is a Polyclonal antibody targeting c-MYC in WB, IF\/ICC, FC (Intra), IP, ELISA applications with reactivity to human samples\n10828-1-AP targets c-MYC in WB, IF\/ICC, FC (Intra), IP, CoIP, chIP, RIP, ELISA, EMSA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HT-29 cells, Jurkat cells,  HeLa cells,  A549 cells,  HL-60 cells,  human placenta tissue,  Raji cells,  SH-SY5Y cells,  HepG2 cells,  BxPC-3 cells,  SW 1990 cells,  Jurkat cells and Raji cells.\nPositive IP detected in: MCF-7 cells\nPositive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: U2OS cells, HEK-293 cells,  Saos-2 cells\nPositive FC (Intra) detected in: HeLa cells, NCCIT cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:12000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nFunctionc-Myc (also known as Myc), together with l-Myc and n-Myc, belongs to the Myc family of transcription factors. c-Myc has a basic helix-loop-helix leucine zipper domain and through heterodimerization can bind and regulate the transcriptional activity of genes, either by repression or activation. It is a key player in the regulation of cell growth and cell cycle progression and acts as a proto-oncogene.Tissue specificityc-Myc is ubiquitously expressed in almost all cell types and its expression positively correlates with tissue proliferative capacity. c-Myc is also expressed during embryogenesis and is upregulated in many cancer types.Involvement in disease·Upregulated in many cancer types, especially in aggressive, poorly differentiated tumors.·Mutations in the MYC gene and breakpoint translocations within the MYC gene cause Burkitt lymphoma.IsoformsThere are 3 different isoforms of c-Myc: c-Myc1, c-Myc2, and c-MycS (PMID: 16260605). They differ in molecular size, can be preferentially expressed during cell growth, and are reported to be functionally distinct.The 50kDa band recognized by antibody is the native form of MYC, while the other bands, between 60-70kDa, are the phosphorylated form of MYC (PMID: 12189186).Post-translational modificationsc-Myc is subject to various post-translational modifications, including phosphorylation, acetylation, and ubiquitinylation (PMID: 16987807), which regulate its activity.Cellular localizationc-Myc localizes to the nucleus but can also be present in the cytoplasm of certain cancer types.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse, rat, pig, chicken, sheep, silkworm, p. lilacinum\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1263 Product name: Recombinant human MYC protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 16-454 aa of BC000141 Sequence: MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA Predict reactive species\nFull Name: v-myc myelocytomatosis viral oncogene homolog (avian)\nCalculated Molecular Weight: 49 kDa\nObserved Molecular Weight: 62-65 kDa, 50 kDa\nGenBank Accession Number: BC000141\nGene Symbol: MYC\nGene ID (NCBI): 4609\nRRID: AB_2148585\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P01106\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868818198697,"sku":"10828-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-10828-1-ap","provider":"Iright","version":"1.0","type":"link"}