{"product_id":"proteintech-10871-1-ap","title":"Proteintech, 10871-1-AP, HOOK1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe HOOK1 (10871-1-AP) by Proteintech is a Polyclonal antibody targeting HOOK1 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse samples\n10871-1-AP targets HOOK1 in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells,  human brain tissue\nPositive IP detected in: mouse brain tissue\nPositive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHOOK1, an orthologue of the Drosophila Hook protein, is a member of the microtubule-binding HOOK family of cytosolic coiled-coil proteins (HOOK1, HOOK2, HOOK3). These proteins contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. HOOK1 is required for spermatid differentiation. It is probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton. HOOK1 is a component of the FTS\/Hook\/FHIP complex which functions to promote vesicle trafficking and\/or fusion via the HOPS (homotypic vesicular protein sorting) complex.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1311 Product name: Recombinant human HOOK1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 377-728 aa of BC011621 Sequence: QVQDLHVKLSSESKRADTLAFEMKRLEEKHEALLKEKERLIEQRDTLKETNEELRCSQVQQDHLNQTDASATKSYENLAAEIMPVEYREVFIRLQHENKMLRLQQEGSENERIEELQEQLEQKHRKMNELETEQRLSKERIRELQQQIEDLQKSLQEQGSKSEGESSSKLKQKLEAHMEKLTEVHEELQKKQELIEDLQPDINQNVQKINELEAALQKKDEDMKAMEERYKMYLEKARNVIKTLDPKLNPASAEIMLLRKQLAEKERRIEILESECKVAKFRDYEEKLIVSAWYNKSLAFQKLGMESRLVSGGGACSDTGACTPARSFLAQQRHITNTRRNLSVKVPATTSD Predict reactive species\nFull Name: hook homolog 1 (Drosophila)\nCalculated Molecular Weight: 85 kDa\nObserved Molecular Weight: 85 kDa\nGenBank Accession Number: BC011621\nGene Symbol: HOOK1\nGene ID (NCBI): 51361\nRRID: AB_2120812\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9UJC3\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868999700649,"sku":"10871-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-10871-1-ap","provider":"Iright","version":"1.0","type":"link"}