{"product_id":"proteintech-10899-1-ap","title":"Proteintech, 10899-1-AP, EIF3E Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe EIF3E (10899-1-AP) by Proteintech is a Polyclonal antibody targeting EIF3E in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples\n10899-1-AP targets EIF3E in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: rat lymph tissue, HeLa cells,  mouse thymus tissue,  mouse pancreas tissue,  Jurkat cells\nPositive IP detected in: Jurkat cells\nPositive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, pig\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1132 Product name: Recombinant human EIF3E protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 145-445 aa of BC008419 Sequence: SGAAEYLYFFRVLVPATDRNALSSLWGKLASEILMQNWDAAMEDLTRLKETIDNNSVSSPLQSLQQRTWLIHWSLFVFFNHPKGRDNIIDLFLYQPQYLNAIQTMCPHILRYLTTAVITNKDVRKRRQVLKDLVKVIQQESYTYKDPITEFVECLYVNFDFDGAQKKLRECESVLVNDFFLVACLEDFIENARLFIFETFCRIHQCISINMLADKLNMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGNNAVSPYQQVIEKTKSLSFRSQMLAMNIEKKLNQNSRSEAPNWATQDSGFY Predict reactive species\nFull Name: eukaryotic translation initiation factor 3, subunit E\nCalculated Molecular Weight: 445 aa, 52 kDa\nObserved Molecular Weight: 48-52 kDa\nGenBank Accession Number: BC008419\nGene Symbol: EIF3E\nGene ID (NCBI): 3646\nRRID: AB_2096898\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P60228\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869404614825,"sku":"10899-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-10899-1-ap","provider":"Iright","version":"1.0","type":"link"}