{"product_id":"proteintech-10970-1-ap","title":"Proteintech, 10970-1-AP, PABPC1,PABP Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PABPC1,PABP (10970-1-AP) by Proteintech is a Polyclonal antibody targeting PABPC1,PABP in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n10970-1-AP targets PABPC1,PABP in WB, IHC, IF\/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HCT 116 cells, mouse testis tissue,  PC-3 cells,  HeLa cells,  A549 cells,  rat testis tissue,  HT-29 cells,  MCF-7 cells,  NCI-H1299 cells,  HCT-116 cells\nPositive IP detected in: mouse testis tissue\nPositive IHC detected in: human pancreas tissue,  human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:5000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nThe poly(A)-binding protein (PABP), which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA, is required for poly(A) shortening and translation initiation [PMID:\n21989405]. Polyadenylate-binding protein 1 (PABPC1) is a cytoplasmic-nuclear shuttling protein important for protein translation initiation, and both RNA processing and stability. In the cytoplasm, PABPC1 binds to the 3' poly(A) tail of eukaryotic mRNAs through its RNA-recognition motifs (RRM) and interacts with the N-terminus of eIF4G, part of the eIF4F complex associated with the 5' cap structure [PMID:20009508, 17381337].\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, pig\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1422 Product name: Recombinant human PABPC1,PABP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 287-636 aa of BC015958 Sequence: RITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAHLTNQYMQRMASVRAVPNPVINPYQPAPPSGYFMAAIPQTQNRAAYYPPSQIAQLRPSPRWTAQGARPHPFQNMPGAIRPAAPRPPFSTMRPASSQVPRVMSTQRVANTSTQTMGPRPAAAAAAATPAVRTVPQYKYAAGVRNPQQHLNAQPQVTMQQPAVHVQGQEPLTASMLASAPPQEQKQMLGERLFPLIQAMHPTLAGKITGMLLEIDNSELLHMLESPESLRSKVDEAVAVLQAHQAKEAAQKAVNSATGVPTV Predict reactive species\nFull Name: poly(A) binding protein, cytoplasmic 1\nCalculated Molecular Weight: 71 kDa\nObserved Molecular Weight: 71 kDa\nGenBank Accession Number: BC015958\nGene Symbol: PABPC1\nGene ID (NCBI): 26986\nRRID: AB_10596918\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P11940\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868847755433,"sku":"10970-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-10970-1-ap","provider":"Iright","version":"1.0","type":"link"}