{"product_id":"proteintech-11083-1-ap","title":"Proteintech, 11083-1-AP, IFT57 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe IFT57 (11083-1-AP) by Proteintech is a Polyclonal antibody targeting IFT57 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat, canine samples\n11083-1-AP targets IFT57 in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, mouse retina tissue,  NIH\/3T3 cells\nPositive IP detected in: HEK-293 cells\nPositive IHC detected in: human prostate cancer tissue, human pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: hTERT-RPE1 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nIFT57, also named as DERP8, ESRRBL1, HIPPI and MHS4R2, belongs to the IFT57 family. It is required for the formation of cilia. IFT57 plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway. It is a component of IFT complex B which composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. In contrast to BBS proteins, IFT57 and IFT88 is found in almost every cilium.(PMID:22072986)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, canine\nCited Reactivity: human, mouse, rat, chicken\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1566 Product name: Recombinant human IFT57 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-429 aa of BC011899 Sequence: MTAALAVVTTSGLEDGVPRSRGEGTGEVVLERGPGAAYHMFVVMEDLVEKLKLLRYEEEFLRKSNLKAPSRHYFALPTNPGEQFYMFCTLAAWLINKAGRPFEQPQEYDDPNATISNILSELRSFGRTADFPPSKLKSGYGEHVCYVLDCFAEEALKYIGFTWKRPIYPVEELEEESVAEDDAELTLNKVDEEFVEEETDNEENFIDLNVLKAQTYHLDMNETAKQEDILESTTDAAEWSLEVERVLPQLKVTIRTDNKDWRIHVDQMHQHRSGIESALKETKGFLDKLHNEITRTLEKISSREKYINNQLENLVQEYRAAQAQLSEAKERYQQGNGGVTERTRLLSEVMEELEKVKQEMEEKGSSMTDGAPLVKIKQSLTKLKQETVEMDIRIGIVEHTLLQSKLKEKSNMTRNMHATVIPEPATGFY Predict reactive species\nFull Name: intraflagellar transport 57 homolog (Chlamydomonas)\nCalculated Molecular Weight: 49 kDa\nObserved Molecular Weight: 49 kDa\nGenBank Accession Number: BC011899\nGene Symbol: IFT57\nGene ID (NCBI): 55081\nRRID: AB_2121957\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9NWB7\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868866072745,"sku":"11083-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-11083-1-ap","provider":"Iright","version":"1.0","type":"link"}