{"product_id":"proteintech-11097-1-ap","title":"Proteintech, 11097-1-AP, c-SRC Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe c-SRC (11097-1-AP) by Proteintech is a Polyclonal antibody targeting c-SRC in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat, hamster samples\n11097-1-AP targets c-SRC in WB, IHC, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, hamster samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HUVEC cells, mouse brain tissue,  COLO 320 cells,  Jurkat cells,  A431 cells,  CHO cells,  SH-SY5Y cells\nPositive IP detected in: SH-SY5Y cells\nPositive IHC detected in: human breast cancer tissue,  human brain tissue,  human spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: A431 cells, MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:100-1:400\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. SRC can be detected as 60 kDa and 55 kDa (PMID: 15047935, 21461171).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat, hamster\nCited Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1575 Product name: Recombinant human c-SRC protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 187-536 aa of BC011566 Sequence: YCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYYSKHADGLCHRLTTVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMSKGSLLDFLKGETGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQYQPGENL Predict reactive species\nFull Name: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)\nCalculated Molecular Weight: 60 kDa\nObserved Molecular Weight: 55 kDa, 60 kDa\nGenBank Accession Number: BC011566\nGene Symbol: SRC\nGene ID (NCBI): 6714\nENSEMBL Gene ID: ENSG00000197122\nRRID: AB_2286705\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P12931\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868830126249,"sku":"11097-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-11097-1-ap","provider":"Iright","version":"1.0","type":"link"}