{"product_id":"proteintech-11255-1-ap","title":"Proteintech, 11255-1-AP, RAD51AP1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe RAD51AP1 (11255-1-AP) by Proteintech is a Polyclonal antibody targeting RAD51AP1 in WB, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n11255-1-AP targets RAD51AP1 in WB, IHC, IF\/ICC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, human brain tissue,  mouse liver tissue,  rat liver tissue,  Raji cells\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHomologous recombination (HR) is an important pathway for the elimination of DNA double-strand breaks (DSBs) and other deleterious lesions from chromosomes. The eukaryotic HR reaction is mediated by RAD51 [PMID:12456786]. RAD51AP1 (RAD51 associated protein 1), so named because it can interact with human RAD51, is a vertebrate-specific protein that enhances the ability of RAD51 to mediate the D-loop reaction. RAD51AP1 knockdown by siRNA leads to cellular sensitivity to DNA damaging agents and elevated DNA damage-induced chromatid breaks. Enhancement of the RAD51 recombinase activity by RAD51AP1 is contingent upon the physical interaction of the two HR factors [PMID:17996710,17996711].\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1781 Product name: Recombinant human RAD51AP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-352 aa of BC016330 Sequence: MVRPVRHKKPVNYSQFDHSDSDDDFVSATVPLNKKSRTAPKELKQDKPKPNLNNLRKEEIPVQEKTPKKRLPEGTFSIPASAVPCTKMALDDKLYQRDLEVALALSVKELPTVTTNVQNSQDKSIEKHGSSKIETMNKSPHISNCSVASDYLDLDKITVEDDVGGVQGKRKAASKAAAQQRKILLEGSDGDSANDTEPDFAPGEDSEDDSDFCESEDNDEDFSMRKSKVKEIKKKEVKVKSPVEKKEKKSKSKCNALVTSVDSAPAAVKSESQSLPKKVSLSSDTTRKPLEIRSPSAESKKPKWVPPAASGGSRSSSSPLVVVSVKSPNQSLRLGLSRLARVKPLHPNATST Predict reactive species\nFull Name: RAD51 associated protein 1\nCalculated Molecular Weight: 38 kDa\nObserved Molecular Weight: 38 kDa\nGenBank Accession Number: BC016330\nGene Symbol: RAD51AP1\nGene ID (NCBI): 10635\nRRID: AB_2300786\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q96B01\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868891599017,"sku":"11255-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-11255-1-ap","provider":"Iright","version":"1.0","type":"link"}