{"product_id":"proteintech-11256-1-ap","title":"Proteintech, 11256-1-AP, Annexin A2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe Annexin A2 (11256-1-AP) by Proteintech is a Polyclonal antibody targeting Annexin A2 in WB, IHC, FC (Intra), IP, ELISA applications with reactivity to human, mouse, rat samples\n11256-1-AP targets Annexin A2 in WB, IHC, IF, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, HeLa cells,  mouse placenta tissue,  THP-1 cells,  NIH\/3T3 cells,  mouse kidney tissue,  rat kidney tissue,  HepG2 cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: mouse placenta tissue, human bowen disease tissue,  human breast cancer tissue,  human hepatocirrhosis tissue,  human liver tissue,  human lung cancer tissue,  human oesophagus cancer tissue,  human prostate cancer tissue,  mouse kidney tissue,  mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive FC (Intra) detected in: K-562 cells, A549 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:500-1:2000\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nAnnexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, pig, monkey, chicken, zebrafish, bovine\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1777 Product name: Recombinant human Annexin A2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-339 aa of BC016774 Sequence: MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD Predict reactive species\nFull Name: annexin A2\nCalculated Molecular Weight: 339 aa, 39 kDa\nObserved Molecular Weight: 35-40 kDa\nGenBank Accession Number: BC016774\nGene Symbol: Annexin A2\nGene ID (NCBI): 302\nENSEMBL Gene ID: ENSG00000182718\nRRID: AB_2057311\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P07355\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868832682153,"sku":"11256-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-11256-1-ap","provider":"Iright","version":"1.0","type":"link"}