{"product_id":"proteintech-11301-1-ap","title":"Proteintech, 11301-1-AP, HARS2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe HARS2 (11301-1-AP) by Proteintech is a Polyclonal antibody targeting HARS2 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples\n11301-1-AP targets HARS2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, human heart tissue,  MCF-7 cells,  mouse kidney tissue,  MDA-MB-231 cells,  rat kidney tissue\nPositive IP detected in: HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHARS2, also named as HARSL, HARSR, HO3 and HisRS, belongs to the class-II aminoacyl-tRNA synthetase family. It is an enzyme that catalyzes the esterification of histidine to its cognate tRNA as an early step in protein biosynthesis. When overexpressed in HIV-1-producing cells, HARS2 was incorporated into wild-type virions but not in ones containing the dilysine-mutated variant of matrix(MA) protein . HO3, through its recognition of MA, plays a role in the life cycle of HIV-1. (PMID: 9445076 ,7755634 ). 1-33aa of HARS2 is transit peptide and this part will be cut in some form(~52kd) of HARS2. This antibody can bind both HARSs for the close sequences.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1827 Product name: Recombinant human HARS2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 157-506 aa of BC014982 Sequence: WRRESPTIVQGRYREFCQCDFDIAGQFDPMIPDAECLKIMCEILSGLQLGDFLIKVNDRRIVDGMFAVCGVPESKFRAICSSIDKLDKMAWKDVRHEMVVKKGLAPEVADRIGDYVQCHGGVSLVEQMFQDPRLSQNKQALEGLGDLKLLFEYLTLFGIADKISFDLSLARGLDYYTGVIYEAVLLQTPTQAGEEPLNVGSVAAGGRYDGLVGMFDPKGHKVPCVGLSIGVERIFYIVEQRMKTKGEKVRTTETQVFVATPQKNFLQERLKLIAELWDSGIKAEMLYKNNPKLLTQLHYCESTGIPLVVIIGEQELKEGVIKIRSVASREEVAIKRENFVAEIQKRLSES Predict reactive species\nFull Name: histidyl-tRNA synthetase 2, mitochondrial (putative)\nCalculated Molecular Weight: 57 kDa\nObserved Molecular Weight: 50-55 kDa\nGenBank Accession Number: BC014982\nGene Symbol: HARS2\nGene ID (NCBI): 23438\nRRID: AB_10640530\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P49590\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869410185385,"sku":"11301-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-11301-1-ap","provider":"Iright","version":"1.0","type":"link"}