{"product_id":"proteintech-11344-1-ap","title":"Proteintech, 11344-1-AP, HDGF Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe HDGF (11344-1-AP) by Proteintech is a Polyclonal antibody targeting HDGF in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human samples\n11344-1-AP targets HDGF in WB, IHC, IF\/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells, HEK-293T cells,  Hela cells\nPositive IP detected in: HepG2 cells\nPositive IHC detected in: human lung cancer tissue, human ovary tumor tissue,  human liver cancer tissue,  human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HEK-293 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHDGF, also named as HMG1L2, belongs to the HDGF family. HDGF is a mitogen for many cell types with nuclear localization necessary for its mitogenic activity. HDGF has also been identified as an important prognostic marker in pathologic cell growth, as it is overexpressed in a number of cancers with expression linked to a poor outcome in lung, esophageal, pancreatic and hepatic cancer.HDGF is highly expressed in human NSCLC tissues, predicting worse prognosis in resected NSCLCs. It might be useful molecular biomarker for predicting the prognosis of resected NSCLCs.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1893 Product name: Recombinant human HDGF protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-240 aa of BC018991 Sequence: MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCVEEPEPEPEAAEGDGDKKGNAEGSSDEEGKLVIDEPAKEKNEKGALKRRAGDLLEDSPKRPKEAENPEGEEKEAATLEVERPLPMEVEKNSTPSEPGSGRGPPQEEEEEEDEEEEATKEDAEAPGIRDHESL Predict reactive species\nFull Name: hepatoma-derived growth factor (high-mobility group protein 1-like)\nCalculated Molecular Weight: 26 kDa\nObserved Molecular Weight: 40-45 kDa\nGenBank Accession Number: BC018991\nGene Symbol: HDGF\nGene ID (NCBI): 3068\nRRID: AB_2232950\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P51858\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868865941673,"sku":"11344-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-11344-1-ap","provider":"Iright","version":"1.0","type":"link"}