{"product_id":"proteintech-11358-1-ap","title":"Proteintech, 11358-1-AP, Harmonin Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe Harmonin (11358-1-AP) by Proteintech is a Polyclonal antibody targeting Harmonin in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n11358-1-AP targets Harmonin in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, PC-3 cells,  mouse brain tissues,  rat brain tissues\nPositive IP detected in: HEK-293 cells\nPositive IHC detected in: Human Colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:8000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:400-1:1600\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nHarmonin, also known as USH1C, is a scaffold protein which interacts with other USH1 proteins (USH1B\/myosin VIIa and USH1G\/sans) to form complex and is implicated in proper development, morphogenesis, and structural integrity of the hair cell mechanoreceptor. Mutations of Harmonin cause Usher syndrome type 1 (congenital deafness and balance disorder, and early-onset sight loss).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1919 Product name: Recombinant human USH1C protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-392 aa of BC016057 Sequence: MDRKVAREFRHKVDFLIENDAEKDYLYDVLRMYHQTMDVAVLVGDLKLVINEPSRLPLFDAIRPLIPLKHQVEYDQLTPRRSRKLKEVRLDRLHPEGLGLSVRGGLEFGCGLFISHLIKGGQADSVGLQVGDEIVRINGYSISSCTHEEVINLIRTKKTVSIKVRHIGLIPVKSSPDEPLTWQYVDQFVSESGGVRGSLGSPGNRENKEKKVFISLVGSRGLGCSISSGPIQKPGIFISHVKPGSLSAEVGLEIGDQIVEVNGVDFSNLDHKEGRELFMTDRERLAEARQRELQRQELLMQKRLAMESNKILQEQQEMERQRRKEIAQKAAEENERYRKEMEQIVEEEEKFKKQWEEDWGSKEQLLLPKTITAEVHPVPLRKPKYDQGVEPE Predict reactive species\nFull Name: Usher syndrome 1C (autosomal recessive, severe)\nCalculated Molecular Weight: 552 aa, 62 kDa\nObserved Molecular Weight: 68-73 kDa\nGenBank Accession Number: BC016057\nGene Symbol: USH1C\/Harmonin\nGene ID (NCBI): 10083\nRRID: AB_2214137\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9Y6N9\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869547843753,"sku":"11358-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-11358-1-ap","provider":"Iright","version":"1.0","type":"link"}