{"product_id":"proteintech-11383-1-ap","title":"Proteintech, 11383-1-AP, SPRY2 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe SPRY2 (11383-1-AP) by Proteintech is a Polyclonal antibody targeting SPRY2 in WB, IHC, IF\/ICC, ELISA applications with reactivity to human, mouse, rat samples\n11383-1-AP targets SPRY2 in WB, IHC, IF\/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: SW 1990 cells, Jurkat cells,  A375 cells,  Neuro-2a cells\nPositive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: A375 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:10-1:100\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSprouty(SPRY) genes encode intracellular inhibitors of receptor tyrosine kinase (RTK) signaling pathways, including those triggered by fibroblast growth factors (Fgfs).  In addition to its well-reported function as an inhibitor of the RAS\/RAF\/MEK signalling cascade, SPRY2 exerts its inhibitory role in the PI3K\/AKT signalling pathway through PTEN, and inhibits ERK\/MAPK activity via EGFR trafficking. SPRY2 was detected 60-65 kDa (PMID:29291435).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag1918 Product name: Recombinant human SPRY2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-315 aa of BC015745 Sequence: MEARAQSGNGSQPLLQTPRDGGRQRGEPDPRDALTQQVHVLSLDQIRAIRNTNEYTEGPTVVPRPGLKPAPRPSTQHKHERLHGLPEHRQPPRLQHSQVHSSARAPLSRSISTVSSGSRSSTRTSTSSSSSEQRLLGSSFSSGPVADGIIRVQPKSELKPGELKPLSKEDLGLHAYRCEDCGKCKCKECTYPRPLPSDWICDKQCLCSAQNVIDYGTCVCCVKGLFYHCSNDDEDNCADNPCSCSQSHCCTRWSAMGVMSLFLPCLWCYLPAKGCLKLCQGCYDRVNRPGCRCKNSNTVCCKVPTVPPRNFEKPT Predict reactive species\nFull Name: sprouty homolog 2 (Drosophila)\nCalculated Molecular Weight: 35 kDa\nObserved Molecular Weight: 35-40 kDa, 60~65 kDa\nGenBank Accession Number: BC015745\nGene Symbol: SPRY2\nGene ID (NCBI): 10253\nRRID: AB_2195287\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O43597\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868960968873,"sku":"11383-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-11383-1-ap","provider":"Iright","version":"1.0","type":"link"}