{"product_id":"proteintech-11525-1-ap","title":"Proteintech, 11525-1-AP, MCC Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe MCC (11525-1-AP) by Proteintech is a Polyclonal antibody targeting MCC in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse samples\n11525-1-AP targets MCC in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: fetal human brain tissue, HeLa cells,  human brain tissue,  L02 cells,  mouse liver tissue\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMCC (mutated in colorectal cancers) gene been previously been identified by virtue of its mutation in human colorectal tumors. As a candidate for the putative colorectal tumor suppressor gene located at 5q21, MCC protein suppresses cell proliferation a3nd the Wnt\/b-catenin pathway in colorectal cancer cells. In addition, MCC is involved in cell migration via binding with Srib and b-catenin,\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag2089 Product name: Recombinant human MCC protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 480-829 aa of BC018919 Sequence: AGVGSSPGDQSGDENITQMLKRAHDCRKTAENAAKALLMKLDGSCGGAFAVAGCSVQPWESLSSNSHTSTTSSTASSCDTEFTKEDEQRLKDYIQQLKNDRAAVKLTMLELESIHIDPLSYDVKPRGDSQRLDLENAVLMQELMAMKEEMAELKAQLYLLEKEKKALELKLSTREAQEQAYLVHIEHLKSEVEEQKEQRMRSLSSTSSGSKDKPGKECADAASPALSLAELRTTCSENELAAEFTNAIRREKKLKARVQELVSALERLTKSSEIRHQQSAEFVNDLKRANSNLVAAYEKAKKKHQNKLKKLESQMMAMVERHETQVRMLKQRIALLEEENSRPHTNETSL Predict reactive species\nFull Name: mutated in colorectal cancers\nCalculated Molecular Weight: 829 aa, 93 kDa\nObserved Molecular Weight: 98 kDa, 113-118 kDa\nGenBank Accession Number: BC018919\nGene Symbol: MCC\nGene ID (NCBI): 4163\nRRID: AB_2143796\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P23508\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868970045609,"sku":"11525-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-11525-1-ap","provider":"Iright","version":"1.0","type":"link"}