{"product_id":"proteintech-11539-1-ap","title":"Proteintech, 11539-1-AP, MAGED1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe MAGED1 (11539-1-AP) by Proteintech is a Polyclonal antibody targeting MAGED1 in WB, IHC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples\n11539-1-AP targets MAGED1 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells,  Apoptosised HeLa cells,  HeLa cells,  human brain tissue\nPositive IHC detected in: human gliomas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive FC (Intra) detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:20-1:200\nFlow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension\n\u003cb\u003eBackground Information\u003c\/b\u003e\nMAGED1 (also known as NRAGE or Dlxin-1) is a member of the type II melanoma antigen (MAGE) family of proteins. MAGED1 can interact with the nerve growth factor receptor p75NTR, antagonize the association of p75NTR with TrkA, inhibit cell cycle progression, and facilitate p75NTR-mediated apoptosis (PMID: 10985348). It has been reported that MAGED1 binds Dlx5 and regulates its transcriptional function. In vivo, MAGED1 binds not only Dlx5 but also Dlx7 and Msx2 and forms homomultimers (PMID: 11084035). MAGED1 knockout mice show depressive behavior and impairments of circadian rhythm (PMID: 20300063; 23314527).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag2109 Product name: Recombinant human MAGED1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 490-834 aa of BC014070 Sequence: PIPPDWQNLRPSPNLRPSPNSRASQNPGAAQPRDVALLQERANKLVKYLMLKDYTKVPIKRSEMLRDIIREYTDVYPEIIERACFVLEKKFGIQLKEIDKEEHLYILISTPESLAGILGTTKDTPKLGLLLVILGVIFMNGNRASEAVLWEALRKMGLRPGVRHPLLGDLRKLLTYEFVKQKYLDYRRVPNSNPPEYEFLWGLRSYHETSKMKVLRFIAEVQKRDPRDWTAQFMEAADEALDALDAAAAEAEARAEARTRMGIGDEAVSGPWSWDDIEFELLTWDEEGDFGDPWSRIPFTFWARYHQNARSRFPQTFAGPIIGPGGTASANFAANFGAIGFFWVE Predict reactive species\nFull Name: melanoma antigen family D, 1\nCalculated Molecular Weight: 86 kDa; 92 kDa\nObserved Molecular Weight: 66 kDa, 92-95 kDa\nGenBank Accession Number: BC014070\nGene Symbol: MAGED1\nGene ID (NCBI): 9500\nRRID: AB_2138545\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q9Y5V3\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869706375337,"sku":"11539-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-11539-1-ap","provider":"Iright","version":"1.0","type":"link"}