{"product_id":"proteintech-11733-1-ap","title":"Proteintech, 11733-1-AP, CUX1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe CUX1 (11733-1-AP) by Proteintech is a Polyclonal antibody targeting CUX1 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n11733-1-AP targets CUX1 in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: Jurkat cells, human brain tissue,  human liver tissue,  mouse liver tissue,  rat brain tissue,  mouse brain tissue,  L02 cells,  rat liver tissue\nPositive IP detected in: mouse brain tissue\nPositive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: SH-SY5Y cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:2000-1:16000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:1000-1:4000\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nCUX1 is a transcription factors that regulates a large number of genes and microRNAs involved in multiple cellular processes, such as DNA replication, progression into S phase and later, the spindle assembly checkpoint that controls progression through mitosis. Cux1, a marker of supragranular layers. CUX1 acts by preventing binding of positively-acting CCAAT factors to promoters to mediated its gene reperssion. Also it's a component of nf-munr repressor, and binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. It can represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. CUX1 protein containing three cut repeats and a homeodomain called p200, there are several truncated isoforms that have been identified. These isoforms include a testis-specific isoform containing one cut repeat and the homeodomain called p55. In situ hybridization showed that mRNA for p55 was most abundant in round spermatids. Additional isoforms include p75, a protein similar in structure to p55, derived from the use of an alternate promoter in intron 20 ; the cut alternately spliced protein (CASP), a Golgi protein that contains amino-terminal sequences but none of the cut repeats or homeodomains ; and several other isoforms (p80, p90, p110, and p150) that appear to arise via proteolytic processing ]. This antibody can recognizes full-length p200 Cux1 and CASP protein (70~80kDa).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, sheep\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag2335 Product name: Recombinant human CUX1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-341 aa of BC025422 Sequence: MAANVGSMFQYWKRFDLQQLQRELDATATVLANRQDESEQSRKRLIEQSREFKKNTPEDLRKQVAPLLKSFQGEIDALSKRSKEAEAAFLNVYKRLIDVPDPVPALDLGQQLQLKVQRLHDIETENQKLRETLEEYNKEFAEVKNQEVTIKALKEKIREYEQTLKNQAETIALEKEQKLQNDFAEKERKLQETQMSTTSKLEEAEHKVQSLQTALEKTRTELFDLKTKYDEETTAKADEIEMIMTDLERANQRAEVAQREAETLREQLSSANHSLQLASQIQKAPDVAIEVLTRSSLEVELAAKEREIAQLVEDVQRLQASLTKLRENSASQISQLEQQLS Predict reactive species\nFull Name: cut-like homeobox 1\nCalculated Molecular Weight: 676 aa, 77 kDa\nObserved Molecular Weight: 80 kDa\nGenBank Accession Number: BC025422\nGene Symbol: CUX1\nGene ID (NCBI): 1523\nRRID: AB_2086995\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q13948\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868832190633,"sku":"11733-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-11733-1-ap","provider":"Iright","version":"1.0","type":"link"}