{"product_id":"proteintech-11840-1-ap","title":"Proteintech, 11840-1-AP, SDCCAG1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe SDCCAG1 (11840-1-AP) by Proteintech is a Polyclonal antibody targeting SDCCAG1 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples\n11840-1-AP targets SDCCAG1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, Jurkat cells\nPositive IP detected in: A549 cells\nPositive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:250-1:1000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nSDCCAG1, also named as NEMF, is a nuclear export mediator. It can be bound to translocon-engaged 60S subunits on native ER membranes. Human SDCCAG1 has been implicated in lung cancers and it may function as a tumor suppressor gene. SDCCAG1 inactivation contributes to the transformed state of human lung cancer cells (PMID:16103875, 10575219). NEMF is also regarded as a potential biomarker for the detection of active and latent TB infections (PMID: 26818387).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag2467 Product name: Recombinant human SDCCAG1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 48-397 aa of BC020794 Sequence: EDITLQSGRDELNEELIQEESSEDEGEYEEVRKDQDSVGEMKDEGEETLNYPDTTIDLSHLQPQRSIQKLASKEESSNSSDSKSQSRRHLSAKERREMKKKKLPSDSGDLEALEGKDKEKESTVHIETHQNTSKNVAAVQPMKRGQKSKMKKMKEKYKDQDEEDRELIMKLLGSAGSNKEEKGKKGKKGKTKDEPVKKQPQKPRGGQRVSDNIKKETPFLEVITHELQDFAVDDPHDDKEEQDLDQQGNEENLFDSLTGQPHPEDVLLFAIPICAPYTTMTNYKYKVKLTPGVQKKGKAAKTALNSFMHSKEATAREKDLFRSVKDTDLSRNIPGKVKVSAPNLLNVKRK Predict reactive species\nFull Name: serologically defined colon cancer antigen 1\nCalculated Molecular Weight: 1076 aa, 123 kDa\nObserved Molecular Weight: 130-140 kDa\nGenBank Accession Number: BC020794\nGene Symbol: SDCCAG1\nGene ID (NCBI): 9147\nRRID: AB_2183413\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O60524\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868908572841,"sku":"11840-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-11840-1-ap","provider":"Iright","version":"1.0","type":"link"}