{"product_id":"proteintech-11965-1-ap","title":"Proteintech, 11965-1-AP, EMG1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe EMG1 (11965-1-AP) by Proteintech is a Polyclonal antibody targeting EMG1 in WB, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n11965-1-AP targets EMG1 in WB, IF\/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293 cells, HeLa cells,  PC-3 cells\nPositive IP detected in: HeLa cells\nPositive IF\/ICC detected in: HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:6000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nEMG1, also named as NEP1, is a highly conserved protein initially identified as \"Essential for Mitotic Growth\" in yeast. EMG1 is required for maturation of the 18S rRNA and biogenesis of the 40S ribosomal subunit. EMG1 is the methyltransferase in the biosynthesis of m1acp3-Psi in eukaryotic 18S rRNAs. It has an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity. A mutation in human EMG1 gene causes Bowen-Conradi syndrome (BCS), an autosomal recessive disorder characterized by severely impaired prenatal and postnatal growth, profound psychomotor retardation, and death in early childhood.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, goat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag2575 Product name: Recombinant human EMG1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-244 aa of BC055314 Sequence: MAAPSDGFKPRERSGGEQAQDWDALPPKRPRLGAGNKIGGRRLIVVLEGASLETVKVGKTYELLNCDKHKSILLKNGRDPGEARPDITHQSLLMLMDSPLNRAGLLQVYIHTQKNVLIEVNPQTRIPRTFDRFCGLMVQLLHKLSVRAADGPQKLLKVIKNPVSDHFPVGCMKVGTSFSIPVVSDVRELVPSSDPIVFVVGAFAHGKVSVEYTEKMVSISNYPLSAALTCAKLTTAFEEVWGVI Predict reactive species\nFull Name: EMG1 nucleolar protein homolog (S. cerevisiae)\nCalculated Molecular Weight: 244 aa, 27 kDa\nObserved Molecular Weight: 27 kDa\nGenBank Accession Number: BC055314\nGene Symbol: EMG1\nGene ID (NCBI): 10436\nRRID: AB_874093\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q92979\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868997341353,"sku":"11965-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-11965-1-ap","provider":"Iright","version":"1.0","type":"link"}