{"product_id":"proteintech-12102-1-ap","title":"Proteintech, 12102-1-AP, PBXIP1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe PBXIP1 (12102-1-AP) by Proteintech is a Polyclonal antibody targeting PBXIP1 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human samples\n12102-1-AP targets PBXIP1 in WB, IHC, IF\/ICC, IP, chIP, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: BxPC-3 cells, A549 cells,  HeLa cells\nPositive IP detected in: HeLa cells\nPositive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HepG2 cells, HeLa cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:2000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nPBXIP1 (pre-B-cell leukemia transcription factor-interacting protein 1), also known as HPIP (hematopoietic PBX-interacting protein), is a regulator of pre-B-cell leukemia transcription factors (BPXs) function. PBXIP1 is a nucleo-cytoplasmic shuttling protein, mainly localized in the cytosol and in small amounts in the nucleus (PMID: 10825160). It interacts with PBX1 as well as PBX2 and PBX3. PBXIP1 inhibits the ability of PBX-HOX heterodimers to bind to target sequences and strongly inhibits the transactivation activity of E2A-PBX. PBXIP1 is also reported to be a microtubule-binding protein, which regulates estrogen receptor functions and plays a role in cancer development and progression (PMID: 17043237; 18302941; 23321675). The predicted PBXIP1 protein has a calculated molecular mass of 81 kDa. This polyclonal antibody raised against 1-300aa of human PBXIP1 recognizes endogenous PBXIP1 with an apparent molecular weight of 95-100 kDa. The slow migration of PBXIP1 possibly results from either post-translational modifications or intrinsic SDS-resistant folding of the protein (PMID: 10825160; 17043237).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nCited Reactivity: human, mouse\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag2746 Product name: Recombinant human PBXIP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC016852 Sequence: MASCPDSDNSWVLAGSESLPVETLGPASRMDPESERALQAPHSPSKTDGKELAGTMDGEGTLFQTESPQSGSILTEETEVKGTLEGDVCGVEPPGPGDTVVQGDLQETTVVTGLGPDTQDLEGQSPPQSLPSTPKAAWIREEGRCSSSDDDTDVDMEGLRRRRGREAGPPQPMVPLAVENQAGGEGAGGELGISLNMCLLGALVLLGLGVLLFSGGLSESETGPMEEVERQVLPDPEVLEAVGDRQDGLREQLQAPVPPDSVPSLQNMGLLLDKLAKENQDIRLLQAQLQAQKEELQSLM Predict reactive species\nFull Name: pre-B-cell leukemia homeobox interacting protein 1\nCalculated Molecular Weight: 731 aa, 81 kDa\nObserved Molecular Weight: 95-100 kDa\nGenBank Accession Number: BC016852\nGene Symbol: PBXIP1\nGene ID (NCBI): 57326\nRRID: AB_2160484\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q96AQ6\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868907622569,"sku":"12102-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-12102-1-ap","provider":"Iright","version":"1.0","type":"link"}