{"product_id":"proteintech-12175-1-ap","title":"Proteintech, 12175-1-AP, OXCT1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe OXCT1 (12175-1-AP) by Proteintech is a Polyclonal antibody targeting OXCT1 in WB, IHC, IF\/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples\n12175-1-AP targets OXCT1 in WB, IHC, IF\/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HeLa cells, mouse skeletal muscle tissue,  rat brain tissue,  rat skeletal muscle tissue\nPositive IP detected in: mouse brain tissue\nPositive IHC detected in: human gliomas tissue, mouse heart tissue,  mouse brain tissue,  human prostate cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF\/ICC detected in: HeLa cells, MCF-7 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:5000-1:50000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:300-1:1200\n\u003cb\u003eBackground Information\u003c\/b\u003e\n3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, canine, bovine\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag2818 Product name: Recombinant human SCOT protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 151-520 aa of BC009001 Sequence: GVPAFYTPTGYGTLVQEGGSPIKYNKDGSVAIASKPREVREFNGQHFILEEAITGDFALVKAWKADRAGNVIFRKSARNFNLPMCKAAETTVVEVEEIVDIGAFAPEDIHIPQIYVHRLIKGEKYEKRIERLSIRKEGDGEAKSAKPGDDVRERIIKRAALEFEDGMYANLGIGIPLLASNFISPNITVHLQSENGVLGLGPYPRQHEADADLINAGKETVTILPGASFFSSDESFAMIRGGHVDLTMLGAMQVSKYGDLANWMIPGKMVKGMGGAMDLVSSAKTKVVVTMEHSAKGNAHKIMEKCTLPLTGKQCVNRIITEKAVFDVDKKKGLTLIELWEGLTVDDVQKSTGCDFAVSPKLMPMQQIAN Predict reactive species\nFull Name: 3-oxoacid CoA transferase 1\nCalculated Molecular Weight: 520 aa, 56 kDa\nObserved Molecular Weight: 56 kDa\nGenBank Accession Number: BC009001\nGene Symbol: OXCT1\nGene ID (NCBI): 5019\nRRID: AB_2157444\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: P55809\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868839497897,"sku":"12175-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-12175-1-ap","provider":"Iright","version":"1.0","type":"link"}