{"product_id":"proteintech-12482-1-ap","title":"Proteintech, 12482-1-AP, NRF1\/nuclear respiratory factor 1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe NRF1\/nuclear respiratory factor 1 (12482-1-AP) by Proteintech is a Polyclonal antibody targeting NRF1\/nuclear respiratory factor 1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n12482-1-AP targets NRF1\/nuclear respiratory factor 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: A549 cells, human lung tissue,  HEK-293T cells\nPositive IHC detected in: human stomach tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nNRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1\/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nCited Reactivity: human, mouse, rat, pig, chicken, zebrafish, bovine, goat, duck, ducks\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag3172 Product name: Recombinant human NRF1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 213-522 aa of BC016925 Sequence: LKYSTGRGKPGWGKESCKPIWWPEDIPWANVRSDVRTEEQKQRVSWTQALRTIVKNCYKQHGREDLLYAFEDQQTQTQATATHSIAHLVPSQTVVQTFSNPDGTVSLIQVGTGATVATLADASELPTTVTVAQVNYSAVADGEVEQNWATLQGGEMTIQTTQASEATQAVASLAEAAVAASQEMQQGATVTMALNSEAAAHAVATLAEATLQGGGQIVLSGETAAAVGALTGVQDANGLFMADRAGRKWILTDKATGLVQIPVSMYQTVVTSLAQGNGPVQVAMAPVTTRISDSAVTMDGQAVEVVTLEQ Predict reactive species\nFull Name: nuclear respiratory factor 1\nCalculated Molecular Weight: 522 aa, 56 kDa\nObserved Molecular Weight: 67 kDa, 45 kDa\nGenBank Accession Number: BC016925\nGene Symbol: NRF1\nGene ID (NCBI): 4899\nRRID: AB_2282876\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q16656\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46868828848297,"sku":"12482-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-12482-1-ap","provider":"Iright","version":"1.0","type":"link"}