{"product_id":"proteintech-12498-1-ap","title":"Proteintech, 12498-1-AP, FOX2\/RBM9 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe FOX2\/RBM9 (12498-1-AP) by Proteintech is a Polyclonal antibody targeting FOX2\/RBM9 in WB, IHC, IF\/ICC, IF-P, IP, ELISA applications with reactivity to human, mouse samples\n12498-1-AP targets FOX2\/RBM9 in WB, IHC, IF\/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HEK-293T cells, HepG2 cells,  HEK-293 cells,  Jurkat cells,  NCCIT cells,  HeLa cells,  Neuro-2a cells\nPositive IP detected in: HepG2 cells, K-562 cells\nPositive IHC detected in: human breast cancer tissue, human placenta tissue,  mouse brain tissue,  human ovary tumor tissue,  human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\nPositive IF-P detected in: mouse brain tissue\nPositive IF\/ICC detected in: HepG2 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:3000\nImmunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate\nImmunohistochemistry (IHC): IHC : 1:50-1:500\nImmunofluorescence (IF)-P: IF-P : 1:50-1:500\nImmunofluorescence (IF)\/ICC: IF\/ICC : 1:200-1:800\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several  isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse\nCited Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag3168 Product name: Recombinant human RBM9 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 6-358 aa of BC013115 Sequence: MVTQGNQEPTTTPDAMVQPFTTIPFPPPPQNGIPTEYGVPHTQDYAGQTGEHNLTLYGSTQAHGEQSSNSPSTQNGSLTTEGGAQTDGQQSQTQSSENSESKSTPKRLHVSNIPFRFRDPDLRQMFGGFGFVTFENSADADRAREKLHGTVVEGRKIEVNNATARVMTNKKMVTPYANGWKLSPVVGAVYGPELYAASSFQADVSLGNDAAVPLSGRGGINTYIPLIIPGFPYPTAATTAAAFRGAHLRGRGRTVYGAVRAVPPTAIPAYPGVVYQDGFYGADLYGGYAAYRYAQPATATAATAAAAAVAAYSDGYGRVYTADPYHALAPAASYGVGAVASLYRGGYSRFAPY Predict reactive species\nFull Name: RNA binding motif protein 9\nCalculated Molecular Weight: 390 aa, 41 kDa\nObserved Molecular Weight: 50- 60 kDa\nGenBank Accession Number: BC013115\nGene Symbol: RBM9\nGene ID (NCBI): 23543\nRRID: AB_2877861\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O43251\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46869461532841,"sku":"12498-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-12498-1-ap","provider":"Iright","version":"1.0","type":"link"}