{"product_id":"proteintech-12547-1-ap","title":"Proteintech, 12547-1-AP, RGS1 Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe RGS1 (12547-1-AP) by Proteintech is a Polyclonal antibody targeting RGS1 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples\n12547-1-AP targets RGS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HL-60 cells, mouse small intestine tissue,  HepG2 cells,  NK-92 cells,  rat small intestine tissue\nPositive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:500-1:1000\nImmunohistochemistry (IHC): IHC : 1:50-1:500\n\u003cb\u003eBackground Information\u003c\/b\u003e\nRGS1 (Regulator of G-protein signaling 1) is also named as 1R20, BL34 and IER1. RGS1 encodes a member of the regulator of G-protein signaling family, which can act as a GTPase-activating protein (GAP), increasing the rate of conversion of the GTP to GDP, driving G-protein into its inactive GDP-bound form, hence attenuating or turning off G-protein-coupled receptor signaling (PMID: 8774882). RGS1 is highly expressed in immune cells including T cells, B cells, natural killer (NK) cells, dendritic cells  and monocytes, suggesting a role for RGS1 in immune cell regulation (PMID: 34956194). RGS1 was highly expressed in tumor tissues and correlated with shorter overall survival, which also appeared in several previous studies, including multiple myeloma, melanoma, nonsmall cell lung cancer, gastric cancer , diffuse large B-cell lymphoma  (PMID: 34956194). RGS1 protein, located at the cytoplasm and membrane, is enriched in tumor tissues compared with normal tissues. RGS1 was identified as a HIF-dependent hypoxia target that dampens cell migration and signal transduction (PMID: 24686421).\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human, mouse, rat\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag3315 Product name: Recombinant human RGS1 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 1-196 aa of BC015510 Sequence: MPGMFFSANPKELKGTTHSLLDDKMQKRRPKTFGMDMKAYLRSMIPHLESGMKSSKSKDVLSAAEVMQWSQSLEKLLANQTGQNVFGSFLKSEFSEENIEFWLACEDYKKTESDLLPCKAEEIYKAFVHSDAAKQINIDFRTRESTAKKIKAPTPTCFDEAQKVIYTLMEKDSYPRFLKSDIYLNLLNDLQANSLK Predict reactive species\nFull Name: regulator of G-protein signaling 1\nCalculated Molecular Weight: 196 aa, 22 kDa\nObserved Molecular Weight: 30 kDa\nGenBank Accession Number: BC015510\nGene Symbol: RGS1\nGene ID (NCBI): 5996\nRRID: AB_3085397\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: Q08116\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887783858345,"sku":"12547-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-12547-1-ap","provider":"Iright","version":"1.0","type":"link"}