{"product_id":"proteintech-12577-1-ap","title":"Proteintech, 12577-1-AP, XPOT Polyclonal antibody","description":"Size: 20ul \/ 150ul\nThe XPOT (12577-1-AP) by Proteintech is a Polyclonal antibody targeting XPOT in WB, ELISA applications with reactivity to human samples\n12577-1-AP targets XPOT in WB, ELISA applications and shows reactivity with human samples.\n\u003cb\u003eTested Applications\u003c\/b\u003e\nPositive WB detected in: HepG2 cells, L02 cells,  SMMC-7721 cells,  Y79 cells\n\u003cb\u003eRecommended dilution\u003c\/b\u003e\nWestern Blot (WB): WB : 1:1000-1:4000\n\u003cb\u003eBackground Information\u003c\/b\u003e\nXPOT (also known as Exportin-T) is a key member of the karyopherin-β family of nuclear transport receptors, specifically responsible for the export of a critical class of small non-coding RNAs, transfer RNAs (tRNAs), from the nucleus to the cytoplasm. This process is essential for protein synthesis, as tRNAs must function in the cytoplasm to decode mRNA sequences and deliver amino acids to the growing polypeptide chain. XPOT recognizes mature tRNAs that have been properly processed and aminoacylated in the nucleus, loading them in a RanGTP-dependent manner. Upon translocation through the nuclear pore complex, the hydrolysis of RanGTP to RanGDP in the cytoplasm triggers the release of the tRNA cargo, thereby ensuring that only functional tRNAs are utilized for translation and maintaining the spatial separation of nuclear and cytoplasmic processes.\n\u003cb\u003eSpecification\u003c\/b\u003e\nTested Reactivity: human\nHost \/ Isotype: Rabbit \/ IgG\nClass: Polyclonal\nType: Antibody\nImmunogen: CatNo: Ag3256 Product name: Recombinant human XPOT protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-366 aa of BC020569 Sequence: MDEQALLGLNPNADSDFRQRALAYFEQLKISPDAWQVCAEALAQRTYSDDHVKFFCFQVLEHQVKYKYSELTTVQQQLIRETLISWLQAQMLNPQPEKTFIRNKAAQVFALLFVTEYLTKWPKFFFDILSVVDLNPRGVDLYLRILMAIDSELVDRDVVHTSEEARRNTLIKDTMREQCIPNLVESWYQILQNYQFTNSEVTCQCLEVVGAYVSWIDLSLIANDRFINMLLGHMSIEVLREEACDCLFEVVNKGMDPVDKMKLVESLCQVLQSAGFFSIDQEEDVDFLARFSKLVNGMGQSLIVSWSKLIKNGDIKNAQEALQAIETKVALMLQLLIHEDDDISSNIIGFCYDYLHILKQLTVLSD Predict reactive species\nFull Name: exportin, tRNA (nuclear export receptor for tRNAs)\nCalculated Molecular Weight: 962 aa, 110 kDa\nObserved Molecular Weight: 100-110 kDa\nGenBank Accession Number: BC020569\nGene Symbol: XPOT\nGene ID (NCBI): 11260\nConjugate: Unconjugated\nForm: Liquid\nPurification Method: Antigen affinity purification\nUNIPROT ID: O43592\nStorage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.\nStorage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.","brand":"Proteintech","offers":[{"title":"Default Title","offer_id":46887925252265,"sku":"12577-1-AP","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/ar\/products\/proteintech-12577-1-ap","provider":"Iright","version":"1.0","type":"link"}